| 1. |
Chiharu ISHII, Takeyuki AKITA, Tomonori KIMURA, Shinsuke SAKAI, Masashi MITA, Tomomi IDE, Yoshitaka ISAKA, Kenji HAMASE, Evaluation of individual variation of D-amino acids in human plasma by a two-dimensional LC-MS/MS system and application to the early diagnosis of chronic kidney disease, Analytical Chemistry, 10.1021/acs.analchem.3c05309, 96, 4876-4883, 2024.02. |
| 2. |
Mai OYAIDE, Chiharu ISHII, Takeyuki AKITA, Tomonori KIMURA, Shinsuke SAKAI, Masayuki MIZUI, Masashi MITA, Tomomi IDE, Yoshitaka ISAKA, Kenji HAMASE, Development of a three-dimensional HPLC system for the determination of serine, threonine and allo-threonine enantiomers in the plasma of patients with chronic kidney disease, Journal of Chromatography A, 10.1016/j.chroma.2024.464739, 1719, 464739, 2024.02. |
| 3. |
Chiharu ISHII, Yosuke TOJO, Komei IWASAKI, Akira FUJII, Takeyuki AKITA, Masanobu NAGANO, Masashi MITA, Tomomi IDE, Kenji HAMASE, Development of a two-dimensional LC-MS/MS system for the determination of proline and 4-hydroxyproline enantiomers in biological and food samples, Analytical Sciences, 10.1007/s44211-024-00530-w, in press, 2024.02. |
| 4. |
Hsin-Miao TSAI, Chin-Ling HSIEH, Chiharu ISHII, Takeyuki AKITA, Masashi MITA, Tomomi IDE, Jen-Ai LEE, Kenji HAMASE, Enantioselective analysis of lactate, hydroxybutyrates and malate in human physiological fluids using a three-dimensional HPLC system, Chromatography, 10.15583/jpchrom.2023.015, 44, 105-113, 2023.09. |
| 5. |
Yuri NAGATA, Takeyuki AKITA, Chiharu ISHII, Mai OYAIDE, Masashi MITA, Tomomi IDE, Kenji HAMASE, Development of an enantioselective three-dimensional HPLC system for the determination of alanine, valine, isoleucine, allo-isoleucine and leucine in human plasma and urine, Journal of Pharmaceutical and Biomedical Analysis Open, 10.1016/j.jpbao.2023.100013, 2, 100013, 2023.06. |
| 6. |
Yuri NAGATA, Takeyuki AKITA, Aogu FURUSHO, Chiharu ISHII, Masashi MITA, Kenji HAMASE, Design of novel mixed-mode columns for analysis of amino acids derivatized with 4-fluoro-7-nitro-2,1,3-benzoxadiazole and the evaluation of retention properties, Chromatography, 10.15583/jpchrom.2023.006, 44, 83-87, 2023.04. |
| 7. |
Y. Gonda, A. Matsuda, K. Adachi, C. Ishii, M. Suzuki, A. Osaki, M. Mita, N. Nishizaki, Y. Ohtomo, T. Shimizu, M. Yasui, K. Hamase, J. Sasabe, Mammals sustain amino acid homochirality against chiral conversion by symbiotic microbes, Proceedings of the National Academy of Sciences of the United States of America, 10.1073/pnas.2300817120, 120, e2300817120, 2023.03, Mammals exhibit systemic homochirality of amino acids in L-configurations. While ribosomal protein synthesis requires rigorous chiral selection for L-amino acids, both endogenous and microbial enzymes convert diverse L-amino acids to D-configurations in mammals. However, it is not clear how mammals manage such diverse D-enantiomers. Here, we show that mammals sustain systemic stereo dominance of L-amino acids through both enzymatic degradation and excretion of D-amino acids. Multidimensional high performance liquidchromatography analyses revealed that in blood, humans and mice maintain D-amino acids at less than several percent of the corresponding L-enantiomers, while D-amino acids comprise ten to fifty percent of the L-enantiomers in urine and feces. Germ-free experiments showed that vast majority of D-amino acids, except for D-serine, detected in mice are of microbial origin. Experiments involving mice that lack enzymatic activity to catabolize D-amino acids showed that catabolism is central to the elimination of diverse microbial D-amino acids, whereas excretion into urine is of minor importance under physiological conditions. Such active regulation of amino acid homochirality depends on maternal catabolism during the prenatal period, which switches developmentally to juvenile catabolism along with the growth of symbiotic microbes after birth. Thus, microbial symbiosis largely disturbs homochirality of amino acids in mice, whereas active host catabolism of microbial D-amino acids maintains systemic predominance of L-amino acids. Our findings provide fundamental insight into how the chiral balance of amino acids is governed in mammals and further expand the understanding of interdomain molecular homeostasis in host-microbial symbiosis.. |
| 8. |
C. Ishii, R. Morinaga, M. Takahashi, M. Mita, T. Akita, K. Hamase, Development of a three-dimensional HPLC system for acidic amino acid enantiomers and determination of their amounts in mice lacking D-aspartic acid oxidase activity, Journal of Pharmaceutical and Biomedical Analysis Open, 10.1016/j.jpbao.2023.100004, 1, 100004, 2023.01. |
| 9. |
C. Ishii, T. Akita, M. Mita, R. Konno, K. Hamase, Chiral amino acid analysis in the plasma of B6DAO-/- mice lacking D-amino acid oxidase activity, Chromatography, 10.15583/jpchrom.2023.002, 44, 39-43, 2023.02. |
| 10. |
Hsin-Miao TSAI, Chin-Ling HSIEH, Chiharu ISHII, Takeyuki AKITA, Masashi MITA, Tomomi IDE, Jen-Ai LEE, Kenji HAMASE, Two-dimensional chiral HPLC analysis of lactate, hydroxybutyrates and malate in human plasma, Chromatography, 10.15583/jpchrom.2022.011, 43, 119-126, 2022.10. |
| 11. |
Chiharu Ishii, Noburo Takizawa, Takeyuki Akita, Masashi Mita, Tomomi Ide, Ryuichi Konno, Kenji Hamase, Off-line two-dimensional LC-MS/MS determination of tryptophan enantiomers in mammalian urine and alteration of their amounts in D-amino acid oxidase deficient mice, Journal of Pharmaceutical and Biomedical Analysis, 10.1016/j.jpba.2022.114919, 219, 114919, 2022.06. |
| 12. |
Siew Hwei YAP, Cheng Siang LEE, Aogu FURUSHO, Chiharu ISHII, Syahirah SHAHARUDIN, Nurul Syuhada ZULHAIMI, Adeeba KAMARULZAMAN, Shahrul Bahyah KAMARUZZAMAN, Masashi MITA, Kok Hoong LEONG, Kenji HAMASE, Reena RAJASURIAR, Plasma D-amino acids are associated with markers of immune activation and organ dysfunction in people with HIV, AIDS, 10.1097/QAD.0000000000003207, 36, 911-921, 2022.06. |
| 13. |
Mai OYAIDE, Chiharu ISHII, Akira FUJII, Takeyuki AKITA, Masashi MITA, Masanobu NAGANO, Kenji HAMASE, Enantioselective Determination of Hydroxy Amino Acids in Japanese Traditional Amber Rice Vinegars , Chromatography, 10.15583/jpchrom.2022.006 , 2022.05. |
| 14. |
Yusuke Gonda, Chiharu Ishii, Masashi Mita, Naoto Nishizaki, Yoshiyuki Otomo, Kenji Hamase, Toshiaki Shimizu, Masato Yasui, Jumpei Sasabe, Astrocytic D-amino acid oxidase degrades D-serine in the hindbrain, FEBS Letters, 2022.05. |
| 15. |
Mai Oyaide, Aogu Furusho, Chiharu Ishii, Takeyuki Akita, Masashi Mita, Tomomi Ide, Kenji Hamase, Development of an off-line heart cutting two-dimensional HPLC system for enantioselective analysis of serine, threonine and allo-threonine in human physiological fluids, Journal of Pharmaceutical and Biomedical Analysis, 10.1016/j.jpba.2022.114807, 217, 2022.04. |
| 16. |
Aogu Furusho, Karina Akemy Ikejiri, Chiharu Ishii, Takeyuki Akita, Masashi Mita, Masanobu Nagano, Tomomi Ide, Kenji Hamase, Two-dimensional high-performance liquid chromatographic determination of chiral amino acids in food samples and human physiological fluids using fluorescence derivatization with 4-(N,N-dimethylaminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole, Chromatography, 43, 1, 29-35, 2022.02. |
| 17. |
Chin-Ling HSIEH, Chiharu ISHII, Takeyuki AKITA, Akira FUJII, Kazunori HASHIGUCHI, Masanobu NAGANO, Masashi MITA, Jen-Ai LEE, Kenji HAMASE, Chiral Analysis of Lactate in Various Food Samples Including Japanese Traditional Amber Rice Vinegar and the Developmental Changes During Fermentation Processes, Chromatography, 10.15583/jpchrom.2021.017, 2021.10. |
| 18. |
Sui-Wen Hsiao, Chiharu Ishii, Aogu Furusho, Chin-Ling Hsieh, Yukiko Shimizu, Takeyuki Akita, Masashi Mita, Tadashi Okamura, Ryuichi Konno, Tomomi Ide, Ching-Kuo Lee, Kenji Hamase, Determination of phenylalanine enantiomers in the plasma and urine of mammals and ᴅ-amino acid oxidase deficient rodents using two-dimensional high-performance liquid chromatography , Biochim. Biophys. Acta-Proteins and Proteomics, 10.1016/j.bbapap.2020.140540, 2020.09. |
| 19. |
Yukiko Shimizu, Chiharu Ishii, Rieko Yanobu-Takanashi, Kenta Nakano, Akio Imaike, Masashi Mita, Kenji Hamase, Tadashi Okamura, D-Amino acid oxidase deficiency is caused by a large deletion in the Dao gene in LEA rats, Biochim. Biophys. Acta-Proteins and Proteomics, 10.1016/j.bbapap.2020.140463, 2020.06. |
| 20. |
Chiharu ISHII, Takeyuki AKITA, Masanobu NAGANO, Masashi MITA, Kenji HAMASE, Determination of chiral amino acids in various fermented products using a two-dimensional HPLC-MS/MS system, Chromatography, 10.15583/jpchrom.2019.011, 2019.03. |
| 21. |
Kenji HAMASE, Tatsuhiko IKEDA, Chiharu ISHII, Shoto ISHIGO, Kei MASUYAMA, Takeyuki AKITA, Aogu FURUSHO, Miho TAKAHASHI, Tomomi IDE, Masashi MITA, Determination of trace amounts of chiral amino acids in complicated biological samples using two-dimensional high-performance liquid chromatography with an innovative “shape-fitting” peak identification/quantification method, Chromatography, 10.15583/jpchrom.2018.019, 2018.10. |
| 22. |
Chiharu ISHII, Takeyuki AKITA, Masashi MITA, Tomomi IDE, Kenji HAMASE, Development of an online two-dimensional high-performance liquid chromatographic system in combination with tandem mass spectrometric detection for enantiomeric analysis of free amino acids in human physiological fluid, Journal of Chromatography A, 10.1016/j.chroma.2018.07.076, 1570, 91-98, 2018.07. |
| 23. |
Chiharu ISHII, Tetsuya MIYAMOTO, Shoto ISHIGO, Yurika MIYOSHI, Masashi MITA, Hiroshi HOMMA, Tadashi UEDA, Kenji HAMASE, Two-dimensional HPLC-MS/MS determination of multiple D-amino acid residues in the proteins stored under various pH conditions, Chromatography, 10.15583/jpchrom.2017.009, 38, 65-72, 2017.05. |
