Kyushu University Academic Staff Educational and Research Activities Database
List of Presentations
Doi Hiroyoshi Last modified date:2022.05.02

Assistant Professor / Operating Rooms / Kyushu University Hospital


Presentations
1. Hiroyoshi Doi Taito Matsuda Kinichi Nakashima, Midazolam anesthesia modifies chromatin landscape to promote neural stem cell dormancy, 幹細胞シンポジウム, 2021.05, Accumulating evidence from human and animal studies suggests that early-life repeated exposure to anesthesia is associated with neurocognitive outcomes in later life, however, little is known how anesthesia leads to long-term CNS dysfunctions. Here we show that early-life exposure to midazolam (MDZ), a widely used drug in pediatric anesthesia, persistently alters chromatin accessibility and expression of quiescence-associated genes in neural stem cells (NSCs) in mouse hippocampus. These alterations led to a continuous restriction of NSC proliferation toward adulthood, resulting in reduction of neurogenesis associated with the impairment of hippocampal-dependent memory functions. Moreover, we found that voluntary exercise, a widely accepted neurogenic stimulus, restored hippocampal neurogenesis accompanied by the normalization of MDZ-perturbed transcriptome and ameliorated declined cognitive ability in MDZ-exposed mice.
Our findings thus pave the way for better understanding of how early-life exposure to anesthesia provokes long-lasting adverse effects on neurocognition and development of a novel therapeutic strategy to prevent delayed-onset learning and memory deficits in people who had experienced anesthesia in their early life.
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2. Hiroyoshi Doi Taito Matsuda Ken Yamaura Sumio Hoka Kinichi Nakashima, Effects of neonatal exposure to an anesthetic midazolam on neural stem/progenitor cell behavior in the adult mouse hippocampus, Anesthesiology annual meeting, 2018.10.
3. Hiroyoshi Doi Taito Matsuda Ken Yamaura Sumio Hoka Kinichi Nakashima;, Effects of neonatal exposure to an anesthetic midazolam on neural stem/progenitor cell behavior in the adult mouse hippocampus, Neuroscience , 2019.10.