| Sonoi Rie | Last modified date：2024.06.03 |
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Homepage
https://kyushu-u.elsevierpure.com/en/persons/rie-sonoi
Reseacher Profiling Tool Kyushu University Pure
Phone
092-802-2805
Academic Degree
Doctor of Philosophy in Engineering, Osaka University
Country of degree conferring institution (Overseas)
No
Field of Specialization
Biochemical engineering
ORCID(Open Researcher and Contributor ID)
https://orcid.org/0000-0002-8597-8942
Total Priod of education and research career in the foreign country
00years00months
Outline Activities
Establishment of non-invasive and quantitative evaluation methods based on cell characteristics and behaviors
Research
Research Interests
Membership in Academic Society
- １．Study on quantitative evaluation and regulation of the maturation through the migratory behaviors of human retinal pigment epithelial cells in confluent state (2011-2017).
２．Study on the development of process and quality control technology for inducing differentiation of human iPS cells into retinal pigment epithelial cells for use in the transplantation (2014-2015).
３．Study on the development of quantitative and non-invasive evaluation methods to understand drug toxicity in HepaRG aggregates using Optical Coherence Tomography (OCT) (2018-2021).
４．Study on establishment of quantitative and non-invasive evaluation methods to understand drug toxicity based on the change in the bile canaliculi behaviors of Hepatocytes (2018-2022).
keyword : confluent state, epithelial cells, non-invasive evaluation, image analysis, regenerative medicine, drug discovery
2023.01. - A novel strategy to facilitate uniform epithelial cell maturation using liquid–liquid interfaces
keyword : liquid-liquid interfaces，epithelial cell, uniform maturation, tight junction, cell behavior
2023.04～2025.03.
Papers
| 1. | Rie Sonoi, Masamichi Kamihira, A novel strategy to facilitate uniform epithelial cell maturation using liquid–liquid interfaces, Scientific Reports, 2024.05, [URL], Epithelial tissue forms and maintains a critical barrier function in the body. A novel culture design aimed at promoting uniform maturation of epithelial cells using liquid materials is described. Culturing Madin–Darby canine kidney (MDCK) cells at the liquid–liquid interface yielded reduced migration and stimulated active cell growth. Similar to solid–liquid interfaces, cells cultured on a fibronectin-coated liquid–liquid interface exhibited active migration and growth, ultimately reaching a confluent state. These cells exhibited reduced stress fiber formation and adopted a cobblestone-like shape, which led to their even distribution in the culture vessel. To inhibit stress fiber formation and apoptosis, the exposure of cells on liquid–liquid interfaces to Y27632, a specific inhibitor of the Rho-associated protein kinase (ROCK), facilitated tight junction formation (frequency of ZO-2-positive cells, FZ = 0.73). In Y27632-exposed cells on the liquid–liquid interface, the value obtained by subtracting the standard deviation of the ratio of nucleus densities in each region that compartmentalized a culture vessel from 1, denoted as HLN, was 0.93 ± 0.01, indicated even cell distribution in the culture vessel at t = 72 h. The behavior of epithelial cells on liquid–liquid interfaces contributes to the promotion of their uniform maturation..  |
| 2. | Sonoi, R. and Hagihara Y., Quantitative understanding of HepaRG cells during drug‐induced intrahepatic cholestasis through changes in bile canaliculi dynamics, Pharmacology Research & Perspectives, 10.1002/prp2.960, 2022.05. |
| 3. | Sonoi, R. and Hagihara Y., Tight junction stabilization prevents HepaRG cell death in drug-induced intrahepatic cholestasis, Biology Open, 10.1242/bio.058606, 2021.06. |
| 4. | Sonoi, R., Yamakawa, T., Nakatani, N., Kokubo, M., and Hagihara, Y., Noninvasive Evaluation of HepaRG Aggregates during Drug‐Induced Intrahepatic Cholestasis Using Optical Coherence Tomography, Advanced Biology, 10.1002/adbi.202000198, 5, 2, 2021.02. |
| 5. | Sonoi, R., and Hagihara, Y., Switching of cell fate through the regulation of cell growth during drug-induced intrahepatic cholestasis, Journal of Bioscience and Bioengineering, 10.1016/j.jbiosc.2020.08.004, 130, 6, 659-665, 2020.12. |
| 6. | Sonoi, R., Kim, M.H., Yamada, K., and Kino-oka, M., Phenotypic heterogeneity of human retinal pigment epithelial cells in passaged cell populations, Journal of bioscience and bioengineering, 10.1016/j.jbiosc.2017.03.008, 124, 2, 227-233, 2017.08. |
| 7. | Sonoi, R., Kim, M.H., and Kino-oka, M., Facilitation of uniform maturation of human retinal pigment epithelial cells through collective movement in culture, Journal of bioscience and bioengineering, 10.1016/j.jbiosc.2015.05.019, 121, 2, 220-226, 2016.02. |
| 8. | Sonoi, R., Kim, M.H., and Kino-oka, M., Locational heterogeneity of maturation by changes in migratory behaviors of human retinal pigment epithelial cells in culture, Journal of bioscience and bioengineering, 10.1016/j.jbiosc.2014.05.025, 119, 1, 107-112, 2015.01. |
| 9. | Kim, M.H., Sonoi, R., Yamada, K., Inamori, M., and Kino-oka, M., Analysis of locality of early-stage maturation in confluent state of human retinal pigment epithelial cells, Journal of bioscience and bioengineering, 10.1016/j.jbiosc.2012.02.009, 113, 6, 778-781, 2012.06. |
Presentations
- The society for biotechnology
- The japanese society for regenerative medicine
- Parenteral Drug Association
- The Japanese Society for Biomaterials
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