| Sonoi Rie | Last modified date：2024.06.03 |
Assistant Professor /
Department of Chemical Engineering /
Faculty of Engineering
Papers
| 1. | Rie Sonoi, Masamichi Kamihira, A novel strategy to facilitate uniform epithelial cell maturation using liquid–liquid interfaces, Scientific Reports, 2024.05, [URL], Epithelial tissue forms and maintains a critical barrier function in the body. A novel culture design aimed at promoting uniform maturation of epithelial cells using liquid materials is described. Culturing Madin–Darby canine kidney (MDCK) cells at the liquid–liquid interface yielded reduced migration and stimulated active cell growth. Similar to solid–liquid interfaces, cells cultured on a fibronectin-coated liquid–liquid interface exhibited active migration and growth, ultimately reaching a confluent state. These cells exhibited reduced stress fiber formation and adopted a cobblestone-like shape, which led to their even distribution in the culture vessel. To inhibit stress fiber formation and apoptosis, the exposure of cells on liquid–liquid interfaces to Y27632, a specific inhibitor of the Rho-associated protein kinase (ROCK), facilitated tight junction formation (frequency of ZO-2-positive cells, FZ = 0.73). In Y27632-exposed cells on the liquid–liquid interface, the value obtained by subtracting the standard deviation of the ratio of nucleus densities in each region that compartmentalized a culture vessel from 1, denoted as HLN, was 0.93 ± 0.01, indicated even cell distribution in the culture vessel at t = 72 h. The behavior of epithelial cells on liquid–liquid interfaces contributes to the promotion of their uniform maturation..  |
| 2. | Sonoi, R. and Hagihara Y., Quantitative understanding of HepaRG cells during drug‐induced intrahepatic cholestasis through changes in bile canaliculi dynamics, Pharmacology Research & Perspectives, 10.1002/prp2.960, 2022.05. |
| 3. | Sonoi, R. and Hagihara Y., Tight junction stabilization prevents HepaRG cell death in drug-induced intrahepatic cholestasis, Biology Open, 10.1242/bio.058606, 2021.06. |
| 4. | Sonoi, R., Yamakawa, T., Nakatani, N., Kokubo, M., and Hagihara, Y., Noninvasive Evaluation of HepaRG Aggregates during Drug‐Induced Intrahepatic Cholestasis Using Optical Coherence Tomography, Advanced Biology, 10.1002/adbi.202000198, 5, 2, 2021.02. |
| 5. | Sonoi, R., and Hagihara, Y., Switching of cell fate through the regulation of cell growth during drug-induced intrahepatic cholestasis, Journal of Bioscience and Bioengineering, 10.1016/j.jbiosc.2020.08.004, 130, 6, 659-665, 2020.12. |
| 6. | Sonoi, R., Kim, M.H., Yamada, K., and Kino-oka, M., Phenotypic heterogeneity of human retinal pigment epithelial cells in passaged cell populations, Journal of bioscience and bioengineering, 10.1016/j.jbiosc.2017.03.008, 124, 2, 227-233, 2017.08. |
| 7. | Sonoi, R., Kim, M.H., and Kino-oka, M., Facilitation of uniform maturation of human retinal pigment epithelial cells through collective movement in culture, Journal of bioscience and bioengineering, 10.1016/j.jbiosc.2015.05.019, 121, 2, 220-226, 2016.02. |
| 8. | Sonoi, R., Kim, M.H., and Kino-oka, M., Locational heterogeneity of maturation by changes in migratory behaviors of human retinal pigment epithelial cells in culture, Journal of bioscience and bioengineering, 10.1016/j.jbiosc.2014.05.025, 119, 1, 107-112, 2015.01. |
| 9. | Kim, M.H., Sonoi, R., Yamada, K., Inamori, M., and Kino-oka, M., Analysis of locality of early-stage maturation in confluent state of human retinal pigment epithelial cells, Journal of bioscience and bioengineering, 10.1016/j.jbiosc.2012.02.009, 113, 6, 778-781, 2012.06. |
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