Kyushu University Academic Staff Educational and Research Activities Database
List of Papers
Masamichi Kamihira Last modified date:2021.10.12

Professor / Molecular and Biochemical Systems Engineering / Department of Chemical Engineering / Faculty of Engineering


Papers
1. Xiaoting Fu, Seiichi Ohta, Takahiro Kawakatsu, Masamichi Kamihira, Yasuyuki Sakai, Taichi Ito, Bioinspired perfluorocarbon-based oxygen carriers with concave shape and deformable shell, Advanced Materials Technologies, 10.1002/admt.202100573, 2100573, 2021.10.
2. Kantaro Yoshioka, Akira Ito, Masanobu Horie, Kazushi Ikeda, Sho Kataoka, Keiichiro Sato, Taichi Yoshigai, Hidetoshi Sakurai, Akitsu Hotta, Yoshinori Kawabe, Masamichi Kamihira, Contractile activity of myotubes derived from human induced pluripotent stem cells: a model of Duchenne muscular dystrophy, Cells, 10.3390/cells10102556, 10, 10, 2556, 2021.09.
3. Guan Huang, Yoshinori Kawabe, Kazuki Shirakawa, Tatsuki Akiyama, Masamichi Kamihira, Novel transgenic Chlamydomonas reinhardtii strain with retargetable genomic transgene integration using Cre-loxP system, Journal of Bioscience and Bioengineering, 10.1016/j.jbiosc.2021.07.006, 132, 5, 2021.11.
4. Shinya Masumoto, Akihiko Ono, Akira Ito, Yoshinori Kawabe, Masamichi Kamihira, Hypoxia-responsive expression of vascular endothelial growth factor for induction of angiogenesis in artificial three-dimensional tissues, Journal of Bioscience and Bioengineering, 10.1016/j.jbiosc.2021.06.010, 132, 4, 399-407, 2021.10.
5. Feiyang Zheng, Yoshinori Kawabe, Mai Murakami, Mamika Takahashi, Kyoka Nishihata, Souichiro Yoshida, Akira Ito, Masamichi Kamihira, LINE-1 vectors mediate recombinant antibody gene transfer by retrotransposition in Chinese hamster ovary cells, Biotechnology Journal, 10.1002/biot.202000620, 16, 7, 2000620, 2021.07.
6. Hiroyuki Kitano, Yuki Nagae, Yoshinori Kawabe, Akira Ito, Masamichi Kamihira, Development of a genetically modified hepatoma cell line with heat-inducible high liver function, Cytotechnology, 10.1007/s10616-021-00457-4, 73, 3, 353-362, 2021.06.
7. Kantaro Yoshioka, Akira Ito, Md Arifuzzaman, Taichi Yoshigai, Fangming Fan, Kei-ichiro Sato, Kazunori Shimizu, Yoshinori Kawabe, Masamichi Kamihira, Miniaturized skeletal muscle tissue fabrication for measuring contractile activity, Journal of Bioscience and Bioengineering, 10.1016/j.jbiosc.2020.11.014, 131, 4, 434-441, 2021.04.
8. Yutaka Tahara, Katsuya Obara, Masamichi Kamihira, Calcium carbonate supplementation to chorioallantoic membranes improves hatchability in shell-less chick embryo culture, Journal of Bioscience and Bioengineering, 10.1016/j.jbiosc.2020.11.001, 131, 3, 314-319, 2021.03.
9. Yusuke Fujii, Kengo Higashi, Hiroshi Mizumoto, Masamichi Kamihira, Toshihisa Kajiwara, A bioartificial liver device based on three-dimensional culture of genetically engineered hepatoma cells using hollow fibers, Cytotechnology, 10.1007/s10616-020-00372-0, 72, 2, 227-237, 2020.04.
10. Ming Shi, Yoshinori Kawabe, Akira Ito, Masamichi Kamihira, Targeted knock-in into the OVA locus of chicken cells using CRISPR/Cas9 system with homology-independent targeted integration, Journal of Bioscience and Bioengineering, 10.1016/j.jbiosc.2019.09.011, 129, 3, 363-370, 2020.03.
11. Akira Ito, Ryoji Teranishi, Kazuki Kamei, Masaki Yamaguchi, Akihiko Ono, Shinya Masumoto, Yuto Sonoda, Masanobu Horie, Yoshinori Kawabe, Masamichi Kamihira, Magnetically triggered transgene expression in mammalian cells by localized cellular heating of magnetic nanoparticles, Journal of Bioscience and Bioengineering, 10.1016/j.jbiosc.2019.03.008, 128, 3, 355-364, 2019.04.
12. Kantaro Yoshioka, Akira Ito, Yoshinori Kawabe, Masamichi Kamihira, Novel neuromuscular junction model in 2D and 3D myotubes co-cultured with induced pluripotent stem cell-derived motor neurons, Journal of Bioscience and Bioengineering, 10.1016/j.jbiosc.2019.10.004, 129, 4, 486-493, 2020.04.
13. Xiaoting Fu, Seiichi Ohta, Masamichi Kamihira, Yasuyuki Sakai, Taichi Ito, Size-controlled preparation of microsized perfluorocarbon emulsions as oxygen carriers via the Shirasu porous glass membrane emulsification technique, Langmuir, 10.1021/acs.langmuir.9b00194, 35, 11, 4094-4100, 2019.03.
14. Paerwen Paerhati, Akira Ito, Kantaro Yoshioka, Kaori Iwamoto, Sho Fujiwara, Masanobu Horie, Yoshinori Kawabe, Masamichi Kamihira, Neural differentiation of mouse induced pluripotent stem cells using cadherin gene-engineered PA6 feeder cells, Journal of Bioscience and Bioengineering, 10.1016/j.jbiosc.2018.10.009, 127, 5, 633-640, 2019.05.
15. Md. Arifuzzaman, Akira Ito, Kazushi Ikeda, Yoshinori Kawabe, Masamichi Kamihira, Fabrication of muscle-neuron constructs with improved contractile force generation using C2C12/PC12 coculture system, Tissue Engineering Part A, 10.1089/ten.TEA.2018.0165, 25, 7-8, 563-574, 2019.04.
16. Seiichi Ohta, Kenichiro Hashimoto, Xiaoting Fu, Masamichi Kamihira, Yasuyuki Sakai, Taichi Ito, Development of human-derived hemoglobin–albumin microspheres as oxygen carriers using Shirasu porous glass membrane emulsification, Journal of Bioscience and Bioengineering, 10.1016/j.jbiosc.2018.04.017, 126, 4, 533-539, 2018.10.
17. Xue Wang, Yoshinori Kawabe, Takeshi Hada, Akira Ito, Masamichi Kamihira, Cre-mediated transgene integration in Chinese hamster ovary cells using minicircle DNA vectors, Biotechnology Journal, 10.1002/biot.201800063, 13, 7, 1800063, 2018.05.
18. Apeksha Damania, Anupam Kumar, Arun K. Teotia, Haruna Kimura, Masamichi Kamihira, Hiroyuki Ijima, Shiv K. Sarin, Ashok Kumar, Decellularized liver matrix modified cryogel scaffolds as potential hepatocyte carriers in bioartificial liver support systems and implantable liver constructs, ACS Applied Materials & Interfaces, 10.1021/acsami.7b13727, 10, 1, 114-126, 2018.01.
19. Yoshinori Kawabe, Shinya Komatsu, Shodai Komatsu, Mai Murakami, Akira Ito, Tetsushi Sakuma, Takahiro Nakamura, Takashi Yamamoto, Masamichi Kamihira, Targeted knock-in of an scFv-Fc antibody gene into the hprt locus of Chinese hamster ovary cells using CRISPR/Cas9 and CRIS-PITCh systems, Journal of Bioscience and Bioengineering, 10.1016/j.jbiosc.2017.12.003, 125, 5, 599-605, 2018.05.
20. Jane Marie Tonello, Saori Kawashima, Kazuki Sato, Yoshinori Kawabe, Akira Ito, Masamichi Kamihira, Three-dimensional culture of a genetically modified hepatoma cell line using macroporous gelatin beads, Cytotechnology, 10.1007/s10616-017-0117-0, 69, 6, 925-931, 2017.12.
21. Joyita Sarkar, Jyoti Kumari, Jane Marie Tonello, Masamichi Kamihira, Ashok Kumar, Enhanced hepatic functions of genetically modified mouse hepatoma cells by spheroid culture for drug toxicity screening, Biotechnology Journal, 10.1002/biot.201700274, 12, 10, 1700274, 2017.10.
22. Hideaki Yamamoto, Jane Marie Tonello, Takanori Sambuichi, Yoshinori Kawabe, Akira Ito, Masamichi Kamihira, Characterization of genetically engineered mouse hepatoma cells with inducible liver functions by overexpression of liver-enriched transcription factors, Journal of Bioscience and Bioengineering, 10.1016/j.jbiosc.2017.07.011, 125, 1, 131-139, 2018.01.
23. Xue Wang, Yoshinori Kawabe, Risa Kato, Takeshi Hada, Akira Ito, Yoshimasa Yamana, Masako Kondo, Masamichi Kamihira, Accumulative scFv-Fc antibody gene integration into the hprt chromosomal locus of CHO cells, Journal of Bioscience and Bioengineering, 10.1016/j.jbiosc.2017.05.017, 124, 5, 583-590, 2017.11.
24. Akihiko Ono, Akira Ito, Tomonaga Sato, Masaki Yamaguchi, Taiga Suzuki, Yoshinori Kawabe, Masamichi Kamihira, Hypoxia-responsive transgene expression system using RTP801 promoter and synthetic transactivator fused with oxygen-dependent degradation domain, Journal of Bioscience and Bioengineering, 10.1016/j.jbiosc.2017.02.012, 124, 1, 115-124, 2017.07.
25. Kazushi Ikeda, Akira Ito, Ryusuke Imada, Masanori Sato, Yoshinori Kawabe, Masamichi Kamihira, In vitro drug testing based on contractile activity of C2C12 cells in an epigenetic drug model, Scientific Reports, 10.1038/srep44570, Vol. 7, 44570, 2017.03.
26. Yoshinori Kawabe, Takanori Inao, Shodai Komatsu, Guan Huang, Akira Ito, Takeshi Omasa, Masamichi Kamihira, Improved recombinant antibody production by CHO cells using a production enhancer DNA element with repeated transgene integration at a predetermined chromosomal site, Journal of Bioscience and Bioengineering, 10.1016/j.jbiosc.2016.10.011, 123, 3, 390-397, 2017.03.
27. Momoko Kitaoka, Ayaka Naritomi, Yoshinori Kawabe, Masamichi Kamihira, Noriho Kamiya, Masahiro Goto, Transcutaneous pollinosis immunotherapy using a solid-in-oil nanodispersion system carrying T cell epitope peptide and R848, Bioengineering & Translational Medicine, 10.1002/btm2.10048, Vol. 2, pp. 102-108, 2017.02.
28. Apeksha Damania, Mohsin Hassan, Nana Shirakigawa, Hiroshi Mizumoto, Anupam Kumar, Shiv K. Sarin, Hiroyuki Ijima, Masamichi Kamihira, Ashok Kumar, Alleviating liver failure conditions using an integrated hybrid cryogel based cellular bioreactor as a bioartificial liver support, Scientific Reports, 10.1038/srep40323, Vol. 7, 40323, 2017.01.
29. Masanobu Horie, Anuj Tripathi, Akira Ito, Kawabe Yoshinori, Masamichi Kamihira, Magnetic nanoparticles
Functionalization and manufacturing of pluripotent stem cells, Advanced Structured Materials, 10.1007/978-981-10-3328-5_9, 363-383, 2017.01, Regenerative medicine uses cell alone or in combination with carrier to deliver at the required site for restoring the normal functions of diseased or degenerated tissue. Various strategies to restore tissue functions involve specific cell types, scaffolds and delivery processes that are still in developmental stage. Obtaining sufficient quantity of cells by non-invasive approach for the application in regenerative medicine is still a challenge. Pluripotent stem cells (PSCs), including embryonic stem cells and induced pluripotent stem cells (iPSCs), possess the inherent ability of self-renewal and differentiation into many cell types. In particular, iPSCs are of a special interest because patient-derived iPSCs have the ability to reproduce patient-specific clinical conditions. The development of manufacturing systems for PSCs, including cell culture engineering, is a challenging research field for the clinical application of PSCs such as in regenerative medicine. One of these manufacturing systems uses magnetic nanoparticles which are well known for their application in magnetic resonance imaging and magnetic hyperthermia. Besides, this chapter is focused on the basics of magnetic nanoparticles, its functionalization and further applications of a magnetic force-based cell manufacturing system for pluripotent stem cells. Indeed, we have developed a procedure in which cells are labeled with magnetite cationic liposomes via electrostatic interaction between the positively charged liposomes and the target cells. The culture system may provide a useful tool for studying the behavior of PSCs and an efficient way of PSCs manufacturing for clinical applications..
30. Yoshinori Kawabe, Takuya Shimomura, Shuohao Huang, Suguru Imanishi, Akira Ito, Masamichi Kamihira, Targeted transgene insertion into the CHO cell genome using Cre recombinase-incorporating integrase-defective retroviral vectors, Biotechnology and Bioengineering, 10.1002/bit.25923, 113, 7, 1600-1610, 10.1002/bit.25923, 2016.07.
31. Kazushi Ikeda, Akira Ito, Masanori Sato, Yoshinori Kawabe, Masamichi Kamihira, Improved contractile force generation of tissue-engineered skeletal muscle constructs by IGF-I and Bcl-2 gene transfer with electrical pulse stimulation, Regenerative Therapy, 10.1016/j.reth.2015.12.004, 3, 38-44, 2016.03.
32. Tetsushi Sakuma, Mitsumasa Takenaga, Yoshinori Kawabe, Takahiro Nakamura, Masamichi Kamihira, Takashi Yamamoto, Homologous Recombination-Independent Large Gene Cassette Knock-in in CHO Cells Using TALEN and MMEJ-Directed Donor Plasmids, International Journal of Molecular Science, 10.3390/ijms161023849, Vol. 16, No. 10, pp. 23849–23866, 2015.10.
33. Kazushi Ikeda, Akira Ito, Masanori Sato, Shota Kanno, Yoshinori Kawabe, Masamichi Kamihira, Effects of heat stimulation and l-ascorbic acid 2-phosphate supplementation on myogenic differentiation of artificial skeletal muscle tissue constructs, Journal of Tissue Engineering and Regenerative Medicine, 10.1002/term.2030, 11, 5, 1322-1331, 10.1002/term.2030, 2017.05.
34. Momoko Kitaoka, Yoko Shin, Noriho Kamiya, Yoshinori Kawabe, Masamichi Kamihira, Masahiro Goto, Transcutaneous Peptide Immunotherapy of Japanese Cedar Pollinosis Using Solid-in-Oil Nanodispersion Technology, AAPS PharmSciTech, 10.1208/s12249-015-0333-x, Vol. 16, No. 6, pp. 1418-1424, 2015.06.
35. Akihiko Ono, Akira Ito, Taiga Suzuki, Masaki Yamaguchi, Yoshinori Kawabe, Masamichi Kamihira, DNA damage-responsive transgene expression mediated by the p53 promoter with transcriptional amplification, Journal of Bioscience and Bioengineering, 10.1016/j.jbiosc.2015.02.009, Vol. 120, No. 4, pp. 463-466, 2015.03.
36. Takanori Inao, Yoshinori Kawabe, Takuro Yamashiro, Yujiro Kameyama, Xue Wang, Akira Ito, Masamichi Kamihira, Improved transgene integration into the Chinese hamster ovary cell genome using the Cre-loxP system, Journal of Bioscience and Bioengineering, 10.1016/j.jbiosc.2014.11.019, 120, 1, 99-106, doi: 10.1016/j.jbiosc.2014.11.019, 2015.01.
37. Masanobu Horie, Akira Ito, Takeshi Maki, Yoshinori Kawabe, Masamichi Kamihira, Magnetically labeled feeder system for mouse pluripotent stem cell culture, Journal of Bioscience and Bioengineering, 10.1016/j.jbiosc.2014.10.020, doi: 10.1016/j.jbiosc.2014.10.020, 2014.11.
38. Akira Ito, Masahiro Yamamoto, Kazushi Ikeda, Masanori Sato, Yoshinori Kawabe, Masamichi Kamihira, Effects of type IV collagen on myogenic characteristics of IGF-I gene-engineered myoblasts, Journal of Bioscience and Bioengineering, 10.1016/j.jbiosc.2014.10.008, doi: 10.1016/j.jbiosc.2014.10.008, 2014.11.
39. Masanori Sato, Kazushi Ikeda, Shota Kanno, Akira Ito, Kawabe Yoshinori, Masamichi Kamihira, Enhancement of contractile force generation of artificial skeletal muscle tissues by mild and transient heat treatment, Current Pharmaceutical Biotechnology, Vol. 14, pp. 1083-1087, 2014.04, Artificial skeletal muscle tissues composed of cells are expected to be used for applications of regenerative medicine and drug screening. Generally, however, the physical forces generated by tissue-engineered skeletal muscle are lower than those of skeletal muscle tissues found in the body. Local hyperthermia is used for many diseases including muscle injuries. It was recently reported that mild heat treatment improved skeletal muscle functions. In this study, we investigated the effects of mild heat treatment on the tissue-engineered skeletal muscle tissues in vitro. We used magnetite cationic liposomes to label C2C12 myoblast cells magnetically, and constructed densely packed artificial skeletal muscle tissues by using magnetic force. Cell culture at 39°C promoted the differentiation of myoblast cells into myotubes. Moreover, the mild and transient heat treatment improved the contractile properties of artificial skeletal muscle tissue constructs. These findings indicate that the culture method using heat treatment is a useful approach to enhance functions of artificial skeletal muscle tissue..
40. Akira Ito, Yasunori Yamamoto, Masanori Sato, Kazushi Ikeda, Masahiro Yamamoto, Hideaki Fujita, Eiji Nagamori, Kawabe Yoshinori, Masamichi Kamihira, Induction of functional tissue-engineered skeletal muscle constructs by defined electrical stimulation, Scientific Reports, 10.1038/srep04781, 4, 4781, 2014.04, Electrical impulses are necessary for proper in vivo skeletal muscle development. To fabricate functional skeletal muscle tissues in vitro, recapitulation of the in vivo niche, including physical stimuli, is crucial. Here, we report a technique to engineer skeletal muscle tissues in vitro by electrical pulse stimulation (EPS). Electrically excitable tissue-engineered skeletal muscle constructs were stimulated with continuous electrical pulses of 0.3 V/mm amplitude, 4 ms width, and 1 Hz frequency, resulting in a 4.5-fold increase in force at day 14. In myogenic differentiation culture, the percentage of peak twitch force (%Pt) was determined as the load on the tissue constructs during the artificial exercise induced by continuous EPS. We optimized the stimulation protocol, wherein the tissues were first subjected to 24.5%Pt, which was increased to 50–60%Pt as the tissues developed. This technique may be a useful approach to fabricate tissue-engineered functional skeletal muscle constructs..
41. Masaki Yamaguchi, Akira Ito, Akihiko Ono, Kawabe Yoshinori, Masamichi Kamihira, Heat-inducible gene expression system by applying alternating magnetic field to magnetic nanoparticles, ACS Synthetic Biology, 10.1021/sb4000838, Vol. 3, No. 5, pp. 273-279, 2014.05, By combining synthetic biology with nanotechnology, we demonstrate remote controlled gene expression using a magnetic field. Magnetite nanoparticles, which generate heat under an alternating magnetic field, have been developed to label cells. Magnetite nanoparticles and heat-induced therapeutic genes were introduced into tumor xenografts. The magnetically triggered gene expression resulted in tumor growth inhibition. This system shows great potential for controlling target gene expression in a space and time selective manner, and may be used for remote control of cell functions via gene expression..
42. Kazuhiro Yoshida, Yuya Okuzaki, Ken-ichi Nishijima, Kenji Kyogoku, Takashi Yamashita, Kawabe Yoshinori, Makoto Motono, Masamichi Kamihira, Shinji Iijima, Recombinant proteins produced into yolk of genetically manipulated chickens are partly sialylated in N-glycan, Cytotechnology, 10.1007/s10616-013-9613-z, 65, 6, 985-992, Vol. 65, No. 6, pp. 985-992, 2013.12, The transgenic chicken is a candidate for the production of biopharmaceutical proteins with several economic superiorities. In general, the addition of sialic acid at the terminal of N-glycan is important for the bioactivity of biopharmaceuticals including plasma half-life; however, sialic acid has not been detected in the N-glycan of proteins produced in the egg white of genetically manipulated chickens. In this study, the extracellular domain of the TNF receptor and single chain Fv fused to Fc (referred to as TNFR/Fc and scFv/Fc, respectively) were purified from the egg yolk of genetically manipulated chickens and their sialylation in N-glycan was examined. In contrast to the glycan in egg white, yolk-derived proteins were partly sialylated. Lectin blot showed the existence of α2,6-sialic acid on TNFR/Fc, which disappeared with the removal of N-glycan by PNGase. In scFv/Fc, up to 7 % of N-glycan contained sialic acid. Disialyl glycans, which were detected in serum-derived scFv/Fc in a previous study, were not found in the yolk sample. Ovarian follicular tissue, which surrounds growing yolk, expressed several neuraminidases, suggesting the partial truncation of glycan during the yolk transfer process from the blood..
43. Yoshinori KAWABE, Yuuki HAYASHIDA, Kensaku NUMATA, Shota HARADA, Yoshifumi HAYASHIDA, Akira ITO, and Masamichi KAMIHIRA, Oral immunotherapy for pollen allergy using T-cell epitope-containing egg white derived from genetically manipulated chickens, PLOS ONE, 10.1371/journal.pone.0048512, Vol. 7, No. 10, e48512, doi:10.1371/journal.pone.0048512, 2012.10.
44. Akira Ito, Masaki Yamaguchi, Noriaki Okamoto, Yuji Sanematsu, Kawabe Yoshinori, Kazumasa Wakamatsu, Shosuke Ito, Hiroyuki Honda, Takeshi Kobayashi, Eiichi Nakayama, Yasuaki Tamura, Masae Okura, Toshiharu Yamashita, Kowichi Jimbow, Masamichi Kamihira, T-cell receptor repertoires of tumor-infiltrating lymphocytes after hyperthermia using functionalized magnetite nanoparticles, Nanomedicine, 10.2217/nnm.12.142, Vol. 8, No. 6, pp. 891-902, doi:10.2217/nnm.12.142, 2013.06.
45. Shuohao HUANG and Masamichi KAMIHIRA, Development of hybrid viral vectors for gene therapy, Biotechnology Advances, 10.1016/j.biotechadv.2012.10.001, Vol. 31, No. 2, pp. 208-223, doi:10.1016/j.biotechadv.2012.10.001, 2013.03.
46. Masaki YAMAGUCHI, Akira ITO, Noriaki OKAMOTO, Yoshinori KAWABE, and Masamichi KAMIHIRA, Heat-inducible transgene expression system incorporating a positive feedback loop of transcriptional amplification for hyperthermia-induced gene therapy, Journal of Bioscience and Bioengineering, 10.1016/j.jbiosc.2012.05.006, Vol. 114, No. 4, pp. 460-465, 2012.10.
47. Akira ITO, Noriaki OKAMOTO, Masaki YAMAGUCHI, Yoshinori KAWABE, and Masamichi KAMIHIRA, Heat-inducible transgene expression with transcriptional amplification mediated by a transactivator, International Journal of Hyperthermia, 10.3109/02656736.2012.738847, Vol. 28, No. 8, pp. 788-798, doi:10.3109/02656736.2012.738847, 2012.12.
48. Masanori SATO, Akira ITO, Hirokazu AKIYAMA, Yoshinori KAWABE, and Masamichi KAMIHIRA, Effects of B-cell lymphoma 2 gene transfer to myoblast cells on skeletal muscle tissue formation using magnetic force-based tissue engineering, Tissue Engineering Part A, 10.1089/ten.TEA.2011.0728, Vol. 19, No. 1-2, pp. 307-315, doi:10.1089/ten.TEA.2011.0728, 2013.01.
49. Yasunori YAMAMOTO, Akira ITO, Hideaki JITSUNOBU, Katsuya YAMAGUCHI, Yoshinori KAWABE, Hiroshi MIZUMOTO, and Masamichi KAMIHIRA, Hollow fiber bioreactor perfusion culture system for magnetic force-based skeletal muscle tissue engineering, Journal of Chemical Engineering of Japan, 10.1252/jcej.11we237, Vol. 45, No. 5, pp. 348-354, 2012.05.
50. Shuohao HUANG, Yoshinori KAWABE, Akira ITO, and Masamichi KAMIHIRA, Adeno-associated virus Rep-mediated targeting of integrase-defective retroviral vector DNA circles into human chromosome 19, Biochemical and Biophysical Research Communications, 10.1016/j.bbrc.2011.11.059, Vol. 417, No. 1, pp. 78-83, 2012.01.
51. Hirokazu OBAYASHI, Yoshinori KAWABE, Hirokatsu MAKITSUBO, Ryoko WATANABE, Yujiro KAMEYAMA, Shuohao HUANG, Yuta TAKENOUCHI, Akira ITO, and Masamichi KAMIHIRA, Accumulative gene integration into a pre-determined site using Cre/loxP, Journal of Bioscience and Bioengineering, 10.1016/j.jbiosc.2011.10.027, Vol. 113, No. 3, pp. 381-388, 2012.03.
52. Hideaki YAMAMOTO, Yoshinori KAWABE, Akira ITO, and Masamichi KAMIHIRA, Enhanced liver functions in mouse hepatoma cells by induced overexpression of liver-enriched transcription factors, Biochemical Engineering Journal, 10.1016/j.bej.2011.10.004, Vol. 60, pp. 67-73, 2012.01.
53. Daisuke KODAMA, Daisuke NISHIMIYA, Ken-ichi NISHIJIMA, Yuki OKINO, Yujin INAYOSHI, Yasuhiro KOJIMA, Ken-ichiro ONO, Makoto MOTONO, Katsuhide MIYAKE, Yoshinori KAWABE, Kenji KYOGOKU, Takashi YAMASHITA, Masamichi KAMIHIRA, and Shinji IIJIMA, Chicken oviduct-specific expression of transgene by a hybrid ovalbumin enhancer and the Tet expression system, Journal of Bioscience and Bioengineering, 10.1016/j.jbiosc.2011.10.006, Vol. 113, No. 2, pp. 146-153, 2012.02.
54. Masanobu HORIE, Akira ITO, Yoshinori KAWABE, and Masamichi KAMIHIRA, A genetically engineered STO feeder system expressing E-cadherin and leukemia inhibitory factor for mouse pluripotent stem cell culture, Journal of Bioprocessing and Biotechniques, 10.4172/2155-9821.S3-001, S3:001, doi: 10.4172/2155-9821.S3-001, 2011.11.
55. Yoshinori KAWABE, Hirokatsu MAKITSUBO, Yujiro KAMEYAMA, Shuohao HUANG, Akira ITO, and Masamichi KAMIHIRA, Repeated integration of antibody genes into a pre-selected chromosomal locus of CHO cells using an accumulative site-specific gene integration system, Cytotechnology, 10.1007/s10616-011-9397-y, Vol. 64, No. 3, pp. 267-279, 2012.05.
56. Masanori SATO, Akira ITO, Yoshinori KAWABE, Eiji NAGAMORI, and Masamichi KAMIHIRA, Enhanced contractile force generation by artificial skeletal muscle tissues using IGF-I gene-engineered myoblast cells, Journal of Bioscience and Bioengineering, 10.1016/j.jbiosc.2011.05.007, Vol. 112, No. 3, pp. 273-278, 2011.09.
57. Masanobu HORIE, Akira ITO, Takeshi MAKI, Yoshinori KAWABE, and Masamichi KAMIHIRA, Magnetic separation of cells from developing embryoid bodies using magnetite cationic liposomes, Journal of Bioscience and Bioengineering, 10.1016/j.jbiosc.2011.04.011, Vol. 112, No. 2, pp. 184-187, 2011.08.
58. Yasunori YAMAMOTO, Akira ITO, Hideaki FUJITA, Eiji NAGAMORI, Yoshinori KAWABE, and Masamichi KAMIHIRA, Functional evaluation of artificial skeletal muscle tissue constructs fabricated by a magnetic force-based tissue engineering technique, Tissue Engineering Part A, 10.1089/ten.TEA.2010.0312, Vol. 17, No. 1-2, pp. 107-114, 2011.01, 生体内の筋肉は極めて高いエネルギー変換効率を有していることから、筋肉組織をアクチュエータとして用いることが可能と考えられる。これまでに、生体内の筋肉組織や心筋細胞を用いて、アクチュエータへの応用を試みた例はいくつかあるが、細胞数の確保が困難、倫理的な問題といった課題が挙げられた。本研究では、半無限的に増殖可能な筋芽細胞株C2C12細胞と機能性磁性ナノ粒子を用いて人工的に三次元筋組織を作製し、電気刺激に応じて収縮運動する人工筋組織を構築することに成功した。.
59. Masanobu HORIE, Akira ITO, Takehiko KIYOHARA, Yoshinori KAWABE, and Masamichi KAMIHIRA, E-cadherin gene-engineered feeder systems for supporting undifferentiated growth of mouse embryonic stem cells, Journal of Bioscience and Bioengineering, 10.1016/j.jbiosc.2010.06.002, Vol. 110, No. 5, pp. 582-587, 2010.11.
60. Shuohao HUANG, Yoshinori KAWABE, Akira ITO, and Masamichi KAMIHIRA, Cre recombinase-mediated site-specific modification of a cellular genome using an integrase-defective retroviral vector, Biotechnology and Bioengineering, 10.1002/bit.22863, Vol. 107, No. 4, pp. 717-729, 2010.11.
61. Hirokazu AKIYAMA, Akira ITO, Yoshinori KAWABE, and Masamichi KAMIHIRA, Fabrication of angiogenic gene-modified myoblast cell sheets using magnetic tissue engineering techniques, Proceedings of The World Congress on Engineering and Computer Science 2010, pp. 806-810, 2010.10.
62. Hirokazu AKIYAMA, Akira ITO, Masanori SATO, Yoshinori KAWABE, and Masamichi KAMIHIRA, Construction of cardiac tissue rings using a magnetic tissue fabrication technique, International Journal of Molecular Sciences, 10.3390/ijms11082910, Vol. 11, No. 8, pp. 2910-2920, 2010.08.
63. Hideaki FUJITA, Kazunori SHIMIZU, Yasunori YAMAMOTO, Akira ITO, Masamichi KAMIHIRA, and Eiji NAGAMORI, Fabrication of scaffold-free contractile skeletal muscle tissue using magnetite-incorporated myogenic C2C12 cells, Journal of Tissue Engineering and Regenerative Medicine, 10.1002/term.253, Vol. 4, No. 6, pp. 437-443, 2010.08.
64. Yujiro KAMEYAMA, Yoshinori KAWABE, Akira ITO, and Masamichi KAMIHIRA, An accumulative site-specific gene integration system using Cre recombinase-mediated cassette exchange, Biotechnology and Bioengineering, 10.1002/bit.22619, Vol. 105, No. 6, pp. 1106-1114, 2010.04, 組換え酵素Cre-loxPシステムを用いて配列特異的に細胞のゲノム染色体上で複数の目的遺伝子を逐次的に無限回導入することができる技術を開発した。抗体医薬やエリスロポエチンなどのバイオ医薬品は、遺伝子組換え動物細胞培養によって生産されているが、この技術を使用することによって、生産細胞の樹立において、目的遺伝子の高効率な導入と特定染色体上での増幅が行えるため、細胞1個あたりの生産性を劇的に向上させることが期待できる。.
65. Carlos Alberto PENNO, Yoshinori KAWABE, Akira ITO, and Masamichi KAMIHIRA, Production of recombinant human erythropoietin/Fc fusion protein by genetically manipulated chickens, Transgenic Research, 10.1007/s11248-009-9310-z, Vol. 19, No. 2, pp. 187-195, 2010.04.
66. Hirokazu AKIYAMA, Akira ITO, Yoshinori KAWABE, and Masamichi KAMIHIRA, Cell-patterning using PEG-modified magnetite nanoparticles, Journal of Biomedical Materials Research Part A, 10.1002/jbm.a.32313, Vol. 92A, No. 3, pp. 1123-1130, 2010.03.
67. Hirokazu AKIYAMA, Akira ITO, Yoshinori KAWABE, and Masamichi KAMIHIRA, Genetically engineered angiogenic cell sheets using magnetic force-based gene delivery and tissue fabrication techniques, Biomaterials, 10.1016/j.biomaterials.2009.11.017, Vol. 31, No. 6, pp. 1251-1259, 2010.02.
68. Yasunori YAMAMOTO, Masahiro KATO, Akira ITO, Yoshinori KAWABE, Kazunori SHIMIDU, Eiji NAGAMORI, and Masamichi KAMIHIRA, Magnetic force-based tissue engineering of skeletal muscle for bio-actuator, Animal Cell Technology: Basic & Applied Aspects (Proceedings of JAACT2008), Vol. 16, pp. 171-176, 2010.05.
69. Carlos Alberto PENNO, Yoshinori KAWABE, Akira ITO, and Masamichi KAMIHIRA, Production of recombinant human EPO and EPO/Fc fusion proteins by Chinese hamster ovary cells, Animal Cell Technology: Basic & Applied Aspects (Proceedings of JAACT2008), Vol. 16, pp. 197-202, 2010.05.
70. Yujiro KAMEYAMA, Yoshinori KAWABE, Akira ITO, and Masamichi KAMIHIRA, Retroviral vectors pseudotyped with chimeric vesicular stomatitis virus glycoprotein for antibody-dependent gene transduction, Animal Cell Technology: Basic & Applied Aspects (Proceedings of JAACT2008), Vol. 16, pp. 185-190, 2010.05.
71. Yoshinori KAWABE, Yoshifumi HAYASHIDA, Kensaku NUMATA, Akira HISHIGAE, Akira ITO, and Masamichi KAMIHIRA, Production of therapeutic proteins composed of seven dominant human T cell epitopes derived from the Japanese cedar pollen allergens, Animal Cell Technology: Basic & Applied Aspects (Proceedings of JAACT2008), Vol. 16, pp. 209-214, 2010.05.
72. Yoshinori KAWABE, Kensaku NUMATA, Masashi TERAMORI, Akira ITO, and Masamichi KAMIHIRA, Development of oviduct-specific gene expression system for transgenic avian bioreactor, Animal Cell Technology: Basic & Applied Aspects (Proceedings of JAACT2008), Vol. 16, pp. 203-208, 2010.05.
73. Akira ITO, Takehiko KIYOHARA, Yoshinori KAWABE, Hiroyuki IJIMA, and Masamichi KAMIHIRA, Enhancement of hepatocyte function through heterotypic cell-cell interactions using E-cadherin-expressing NIH3T3 cells, Animal Cell Technology: Basic & Applied Aspects (Proceedings of JAACT2008), Vol. 16, pp.159-164, 2010.05.
74. Hirokazu AKIYAMA, Akira ITO, Yoshinori KAWABE, and Masamichi KAMIHIRA, Magnetic cell-patterning for tissue engineering, Animal Cell Technology: Basic & Applied Aspects (Proceedings of JAACT2008), Vol. 16, pp. 165-170, 2010.05.
75. Yasunori YAMAMOTO, Akira ITO, Masahiro KATO, Yoshinori KAWABE, Kazunori SHIMIZU, Hideaki FUJITA, Eiji NAGAMORI, and Masamichi KAMIHIRA, Preparation of artificial skeletal muscle tissues by a magnetic force-based tissue engineering technique, Journal of Bioscience and Bioengineering, 10.1016/j.jbiosc.2009.05.019, Vol. 108, No. 6, pp. 538-543, 2009.12.
76. Akira ITO, Hideaki JITSUNOBU, Yoshinori KAWABE, Hiroyuki IJIMA, and Masamichi KAMIHIRA, Magnetic separation of cells in co-culture systems using magnetite cationic liposomes, Tissue Engineering Part C: Methods, Vol. 15, No. 3, pp. 413-423, 2009.09.
77. Akira ITO, Tetsuya TAKAHASHI, Yoshinori KAWABE, and Masamichi KAMIHIRA, Human beta defensin-3 engineered keratinocyte sheets constructed by a magnetic force-based tissue engineering technique, Journal of Bioscience and Bioengineering, Vol. 108, No. 3, pp. 244-247, 2009.09.
78. Hirokazu AKIYAMA, Akira ITO, Yoshinori KAWABE, and Masamichi KAMIHIRA, Fabrication of complex three-dimensional tissue architectures using a magnetic force-based cell pattering technique, Biomedical Microdevices, Vol. 11, No. 4, pp. 713-721, 2009.08.
79. Masamichi KAMIHIRA, Yoshinori KAWABE, Takuya SHINDO, Ken-ichiro ONO, Kazuhisa ESAKA, Takashi YAMASHITA, Ken-ichi NISHIJIMA, and Shinji IIJIMA, Production of chimeric monoclonal antibodies by genetically manipulated chickens, Journal of Biotechnology, Vol. 141, No. 1-2, pp. 18-25, 2009.04.
80. Akira ITO, Tetsuya TAKAHASHI, Yujiro KAMEYAMA, Yoshinori KAWABE, and Masamichi KAMIHIRA, Magnetic concentration of a retroviral vector using magnetite cationic liposomes, Tissue Engineering Part C: Methods, Vol. 15, No. 1, pp. 57-64, 2009.03.
81. Akira ITO, Hiroyuki HONDA, and Masamichi KAMIHIRA, Construction of Multi-layered Cell Sheet Using Magnetite Nanoparticles and Magnetic Force, Animal Cell Technology: Basic & Applied Aspects (Proceedings of JAACT2006), Vol. 15, pp. 129-136, 2009.10.
82. Yoshinori KAWABE, Tsutomu NAKA, Hiroyuki KOMATSU, Ken-ichi NISHIJIMA, Shinji IIJIMA, and Masamichi KAMIHIRA, Retroviral gene transduction into chicken embryo gonads through blood circulation, Journal of Bioscience and Bioengineering, Vol. 106, No. 6, pp. 598-601, 2008.12.
83. Yujiro KAMEYAMA, Yoshinori KAWABE, Akira ITO, and Masamichi KAMIHIRA, Antibody-dependent gene transduction using gammaretroviral and lentiviral vectors pseudotyped with chimeric vesicular stomatitis virus glycoprotein, Journal of Virological Methods, Vol. 153, No. 1, pp. 49-54, 2008.10.
84. Akira ITO, Takehiko KIYOHARA, Yoshinori KAWABE, Hiroyuki IJIMA, and Masamichi KAMIHIRA, Enhancement of cell fucntion through heterotypic cell-cell interactions using E-cadherin-expressing NIH3T3 cells, Journal of Bioscience and Bioengineering, Vol. 105, No. 6, pp.679-682, 2008.06.
85. Kenji KYOGOKU, Kazuhiro YOSHIDA, Hiroyuki WATANABE, Takashi YAMASHITA, Yoshinori KAWABE, Makoto MOTONO, Ken-ichi NISHIJIMA, Masamichi KAMIHIRA, and Shinji IIJIMA, Production of recombinant tumor necrosis factor receptor/Fc fusion protein by genetically manipulated chickens, Journal of Bioscience and Bioengineering, Vol. 105, No. 5, pp.454-459, 2008.05.
86. Daisuke KODAMA, Daisuke NISHIMIYA, Ken-ichi IWATA, Kazuhisa YAMAGUCHI, Kazuo YOSHIDA, Yoshinori KAWABE, Makoto MOTONO, Hiroyuki WATANABE, Takashi YAMASHITA, Ken-ichi NISHIJIMA, Masamichi KAMIHIRA, and Shinji IIJIMA, Production of human erythropoietin by chimeric chickens, Biochemical and Biophysical Research Communications, Vol.367, No.4, pp.834-839, 2008.03.
87. Akira ITO, Hideaki JITSUNOBU, Yoshinori KAWABE, and Masamichi KAMIHIRA, Construction of heterotypic cell sheets by magnetic force-based 3-D coculture of HepG2 and NIH3T3 cells, Journal of Bioscience and Bioengineering, Vol.104, No.5, pp.371-378, 2007.11.
88. Akira ITO, Hirokazu AKIYAMA, Yoshinori KAWABE, and Masamichi KAMIHIRA, Magnetic force-based cell patterning using Arg-Gly-Asp (RGD) peptide-conjugated magnetite cationic liposomes, Journal of Bioscience and Bioengineering, Vol.104, No.4, pp.288-293, 2007.10.
89. Mahboob MORSHED, Munetoshi ANDO, Junko YAMAMOTO, Akitsu HOTTA, Hidenori KANEOKA, Jun KOJIMA, Ken-ichi NISHIJIMA, Masamichi KAMIHIRA, and Shinji IIJIMA, YY1 binds to regulatory element of chicken lysozyme and ovalbumin promoters, Cytotechnology, 52, 159-170, 2007.04.
90. Yoshinori KAWABE, Tsutomu NAKA, Naoko ANDO-NOUMI, Ken-ichiro ONO, Hiroyuki MATSUMOTO, Ken-ichi NISHIJIMA, Masamichi KAMIHIRA and Shinji IIJIMA, Transport of human immunoglobulin G and Fc-fusion proteins to chicken egg yolk, Journal of Bioscience and Bioengineering, Vol.102, No.6, pp.518-523, 2006.12.
91. Yoshinori KAWABE, Masamichi KAMIHIRA, Ken-ichiro ONO, Kenji KYOGOKU, Ken-ichi NISHIJIMA and Shinji IIJIMA, Production of scFv-Fc fusion protein using genetically manipulated quails, Journal of Bioscience and Bioengineering, Vol.102, No.4, pp.297-303, 2006.10.
92. Yoshiko MIURA, Akio SAKAKI, Masamichi KAMIHIRA, Shinji IIJIMA, and Kazukiyo KOBAYASHI, A globotriaosylceramide (Gb3Cer) mimic peptide isolated from phage display library expressed strong nertralization to Shiga toxins, Biochimica et Biophysica Acta, Vol.1760, No.6, pp.883-889, 2006.06.
93. Katsuhide MIYAKE, Mikio TAKAHASHI, Takeaki DOHDA, Hidenori KANEOKA, Yoshitaka SATO, Yujin INAYOSHI, Masamichi KAMIHIRA and Shinji IIJIMA, Transcriptional regulation of the alpha-fetoprotein gene by SWI/SNF chromatin remodeling complex, Cytotechnology, Vol.49, No.2-3, pp.143-151, 2006.06.
94. Akitsu HOTTA, Yoshikazu SAITO, Kenji KYOGOKU, Yoshinori KAWABE, Ken-ichi NISHIJIMA, Masamichi KAMIHIRA and Shinji IIJIMA, Characterization of transient expression system for retroviral vector production, Journal of Bioscience and Bioengineering, Vol.101, No.4, pp.361-368, 2006.04.
95. Akitsu HOTTA, Yoshikazu SAITO, Ken-ichi NISHIJIMA, Masamichi KAMIHIRA and Shinji IIJIMA, Preparation of high-titer retroviral vector using transient expression system, Animal Cell Technology: Basic & Applied Aspects, Vol.14, pp.293-299, 2006.04.
96. Mahboob MORSHED, Junko YAMAMOTO, Shusuke SANO, Ken-ichi NISHIJIMA, Masamichi KAMIHIRA and Shinji IIJIMA, Biochemical analysis of chicken ovalbumin promoter, Animal Cell Technology: Basic & Applied Aspects, Vol.14, pp.301-307, 2006.04.
97. Yoshinori KAWABE, Akitsu HOTTA, Ken-ichiro ONO, Kazuhisa ESAKA, Ken-ichi NISHIJIMA, Masamichi KAMIHIRA and Shinji IIJIMA, Production of chimeric antibodies by transgenic chicken bioreactor, Animal Cell Technology: Basic & Applied Aspects, Vol.14, pp.307-314, 2006.04.
98. Mikio TAKAHASHI, Takeaki DOHDA, Hidenori KANEOKA, Yoshitaka SATO, Yujin INAYOSHI, Katsuhide MIYAKE, Masamichi KAMIHIRA and Shinji IIJIMA, Transcriptional regulation of alpha-fetoprotein gene in hepatocytes, Animal Cell Technology: Basic & Applied Aspects, Vol.14, pp.361-368, 2006.04.
99. Masamichi KAMIHIRA, Ken-ichiro ONO, Kazuhisa ESAKA, Ken-ichi NISHIJIMA, Ryoko KIGAKU, Hiroyuki KOMATSU, Takashi YAMASHITA, Kenji KYOGOKU, and Shinji IIJIMA, High-level expression of scFv-Fc fusion protein in serum and egg white of genetically manipulated chickens by using a retroviral vector, Journal of Virology, 10.1128/JVI.79.17.10864-10874.2005, 79, 17, 10864-10874, Vol. 79, No. 17, pp. 10864-10874, 2005.09.