九州大学 研究者情報
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基本情報 研究活動 教育活動
藤岡 寛(ふじおか ゆたか) データ更新日:2021.07.27



主な研究テーマ
悪性脳腫瘍に対するliquid biopsy
キーワード:リキッドバイオプシー
2018.04~2021.05.
研究業績
主要原著論文
1. Yutaka Fujioka, Nobuhiro Hata, Yojiro Akagi, Daisuke Kuga, Ryusuke Hatae, Yuhei Sangatsuda, Yuhei Michiwaki, Takeo Amemiya, Kosuke Takigawa, Yusuke Funakoshi, Aki Sako, Toru Iwaki, Koji Iihara & Masahiro Mizoguchi, Molecular diagnosis of diffuse glioma using a chip-based digital PCR system to analyze IDH, TERT, and H3 mutations in the cerebrospinal fluid, Journal of Neuro-Onchology, 2021.01, Purpose
Conventional genetic analyzers require surgically obtained tumor tissues to confirm the molecular diagnosis of diffuse glioma. Recent technical breakthroughs have enabled increased utilization of cell-free tumor DNA (ctDNA) in body fluids as a reliable resource for molecular diagnosis in various cancers. Here, we tested the application of a chip-based digital PCR system for the less invasive diagnosis (i.e., liquid biopsy) of diffuse glioma using the cerebrospinal fluid (CSF).

Methods
CSF samples from 34 patients with diffuse glioma were collected from the surgical field during craniotomy. Preoperative lumbar CSF collection was also performed in 11 patients. Extracted ctDNA was used to analyze diagnostic point mutations in IDH1 R132H, TERT promoter (C228T and C250T), and H3F3A (K27M) on the QuantStudio® 3D Digital PCR System. These results were compared with their corresponding tumor DNA samples.

Results
We detected either of the diagnostic mutations in tumor DNA samples from 28 of 34 patients. Among them, we achieved precise molecular diagnoses using intracranial CSF in 20 (71%). Univariate analyses revealed that the World Health Organization (WHO) grade (p = 0.0034), radiographic enhancement (p = 0.0006), and Mib1 index (p = 0.01) were significant predictors of precise CSF-based molecular diagnosis. We precisely diagnosed WHO grade III or IV diffuse gliomas using lumbar CSF obtained from 6 (87%) of 7 patients with tumors harboring any mutation.

Conclusion
We established a novel, non-invasive molecular diagnostic method using a chip-based digital PCR system targeting ctDNA derived from CSF with high sensitivity and specificity, especially for high-grade gliomas..
主要学会発表等
1. 藤岡寛、秦 暢宏、空閑太亮、赤木洋二郎、波多江龍亮、三月田祐平、瀧川浩介、舟越勇介、溝口昌弘, 髄液中cell free tumor DNAを用いたDiffuse gliomaの分子診断, 一般社団法人日本脳神経外科学会第78回学術総会, 2020.10.
学会活動
所属学会名
日本脳卒中学会
日本脳神経外科学会
研究資金
競争的資金(受託研究を含む)の採択状況
2021年度~2021年度, 公益財団法人ふくおか公衆衛生推進機構 がん研究助成 奨励賞, 代表, 神経膠腫に対するliquid biopsy.

九大関連コンテンツ

pure2017年10月2日から、「九州大学研究者情報」を補完するデータベースとして、Elsevier社の「Pure」による研究業績の公開を開始しました。
 
 
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