九州大学 研究者情報
論文一覧
林 信哉(はやし のぶや) データ更新日:2024.03.29

教授 /  総合理工学研究院 エネルギー科学部門 高密度エネルギー理工学


原著論文
1. Shunta Kimura, Shu Ishikawa, Nobuya Hayashi, Kazuhisa Fujita, Yuko Inatomi, Shino Suzuki, Bacterial and fungal bioburden reduction on material surfaces using various sterilization techniques suitable for spacecraft decontamination, Frontiers in Microbiology, https://doi.org/10.3389/fmicb.2023.1253436, 14, 1253436-01-1253436-14, 2023.12, Planetary protection is a guiding principle aiming to prevent microbial contamination of the solar system by spacecraft (forward contamination) and extraterrestrial contamination of the Earth (backward contamination). Bioburden reduction on spacecraft, including cruise and landing systems, is required to prevent microbial contamination from Earth during space exploration missions. Several sterilization methods are available; however, selecting appropriate methods is essential to eliminate a broad spectrum of microorganisms without damaging spacecraft components during manufacturing and assembly. Here, we compared the effects of different bioburden reduction techniques, including dry heat, UV light, isopropyl alcohol (IPA), hydrogen peroxide (H2O2), vaporized hydrogen peroxide (VHP), and oxygen and argon plasma on microorganisms with different resistance capacities. These microorganisms included Bacillus atrophaeus spores and Aspergillus niger spores, Deinococcus radiodurans, and Brevundimonas diminuta, all important microorganisms for considering planetary protection. Bacillus atrophaeus spores showed the highest resistance to dry heat but could be reliably sterilized (i.e., under detection limit) through extended time or increased temperature. Aspergillus niger spores and D. radiodurans were highly resistant to UV light. .
2. Shameem Ahmed, Sayma Khanom, Nobuya Hayashi, Persistence of Growth Enhancement Induced by Oxygen Plasma Irradiation Seed and Leaf, Agronomy, MDPI, https://doi.org/10.3390/agronomy13061579, 13, 6, 1579-1-1579-11, 2023.06, The variation of gene expression of seeds or leaves of Arabidopsis thaliana was investigated by irradiation with oxygen and air plasmas. The irradiation with oxygen plasma reported on the growth promotion and induced the consequence of gene expression in plant cells by neutral active oxygen species. The increase in leaf area ratio by oxygen plasma to seeds was due to epigenetics such as activation of DNA demethylation transcription factors and the growth enhancement effect induced by the plasma irradiation of seeds was inherited by next-generation cells through cell division even after germination. In oxygen irradiation for 10 s, expression of each de-DNA methylation-related gene increased, and DNA methylation-related genes decreased in expression. DNA acetylation that induces gene expressions was suppressed. However, irradiated for 20 s by oxygen, both demethylation suppression and promotion and methylation/acetylation suppression and promotion were obtained. On the other hand, methylation and demethylation may occur at the same time but were not significant and the acetylation was suppressed by air plasma irradiation. In both cases, active oxygen species was the key factor for the variation of gene expression..
3. Reona Muto, Nobuya Hayashi, Sterilization characteristics of narrow tubing by nitrogen oxides generated in atmospheric pressure air plasma, Scientific Reports, https://doi.org/10.1038/s41598-023-34243-3, 13, 1, 6947-1-6947-11, 2023.04, The sterilization characteristics of active species generated by an atmospheric dielectric barrier discharge plasma using air and oxygen at the inner surface of silicone tubing were investigated. A dielectric barrier discharge torch plasma device was installed at one end of the tube and generated long-lived active species that flowed into the tube. A strip-type biological indicator with a 105-cell bacterial spore was placed at the opposite end of the 60 cm tube. Sterilization was completed within 30 min by active particles generated from the air plasma. The main factors contributing to the sterilization by air plasma were HNO3 and N2O5. When organic materials (keratin, aspartic acid, and dipicolinic acid) reflecting components of the bacterial spore, were treated by the sterilization procedure there was little effect on dipicolinic acid. Keratin was oxidized by ozone and NOx generated from the oxygen and air plasmas, respectively. Aspartic acid underwent little change in composition from ozone generated from the oxygen plasma, whereas nitro (NO2), nitroso (NO), and aldehyde (CHO) groups were formed from ozone and NOx generated from the air plasma..
4. Agnieszka Starek-Wójcicka, Agnieszka Sagan, Piotr Terebun, Michał Kwiatkowski, Emilia Osmólska, Marta Krajewska, Elżbieta Grządka, Narumol Matsuyama, Nobuya Hayashi, Joanna Pawlat, Quality of Tomato Juice as Influenced by Non-Thermal Air Plasma Treatment, Applied Sciences, MDPI, 10.3390/app13010578, 13, 1, 578-1-578-13, 2022.12, This paper presents the results of an experiment based on using a Glide-arc type plasma reactor operating at atmospheric pressure for the quality of fresh pressed tomato juice, variety Bekas. The impact of after-glow plasma gas (air) on the physicochemical, microbiological properties and morphology of the product’s samples was investigated. Five groups of juices characterized by different exposure times (30, 60, 120, 300 and 600 s), as well as untreated juice (as control) were used. The juice quality was assessed on days 1, 3, 5, and 10 of refrigerated storage. Significant increases were observed when Cold Atmospheric Plasma (CAP)-treated tomato juice was tested against total soluble solids, pH, lycopene, and vitamin C in comparison to the control treatments. Moreover, changes in the tested physicochemical values during the storage of juice subjected to the action of cold plasma did not progress as quickly as in the case of the control juice. A significant decrease was observed in total plate count, yeast, and mold after 300–600 s CAP treatment. The findings of the current study suggested that CAP treatment is a promising technique that could provide improved quality and stability during the processing of tomato juice with better physicochemical properties and bioavailable nutrients..
5. Nobuya Hayashi, Haruka Uematsu, Reona Aijima, Yoshio Yamashita, Enhancement of cytokine production and differentiation from sensitized EL4 T-cell by using atmospheric plasma irradiation, Bulletin of the American Physical Society, HT4, 00088, 2022.10, In recent years, immunotherapy for cancer has become popular as an alternative of conventional methods. However, the period required to culture immune cells up to the enough activity for cancer treatment is as long as 3 weeks. To reduce this period, the purpose of this study is to activate immune cells with plasma and enhance the release of cytokines and differentiation from precursor cells. Oxygen plasma generated by the torch-type atmospheric DBD was supplied to the cells. The oxygen gas flows at 0.6 L/m with discharge voltage 4.2 kV. The EL4 T-cell that is one of kinds of lymphocytes ….
6. Nobuya Hayashi, Kyotaro Yamamoto, Variations in Plant Growth Characteristics Due to Oxygen Plasma Irradiation on Leaf and Seed, Agronomy, MDPI, https://doi.org/10.3390/agronomy12020259, 12, 2, 259-1-259-19, 2022.02, Gene expression variations of plant leaf are investigated by irradiating seed and leaf with oxygen or air plasmas. Enhancement of leaf growth is induced by oxygen plasma irradiation on seeds, which is supported by increased gene expression for protein synthesis, oxidative-reduction reactions, and decreased gene expression concerning DNA methylation and histone modification. Suppression of leaf growth is observed by the oxygen plasma, which would be owing to increased gene expression concerning heat shock protein and redox reaction, and decreased expression of photosynthesis and glycoprotein. Moreover, gene expression variation due to air plasma irradiation is almost the same as that of oxygen plasma. Active oxygen species are major factors in both oxygen and air plasmas for the variation of gene expressions in plants..
7. K Takaki, K Takahashi, N Hayashi, D Wang, T Ohshima, Pulsed power applications for agriculture and food processing, Reviews of Modern Plasma Physics, https://doi.org/10.1007/s41614-021-00059-9, 5, 12, 1-112, 2021.12, Recently, pulsed power technologies, including pulsed electric fields (PEFs) and time-modulated plasmas, are starting to be applied actively in agriculture and food processing. In the applications, compact pulsed power generators with moderate peak power and repetitive operation are developed for controlling discharge plasmas and electric field distribution. These applications are mainly based on the biological effects of a spatially distributed electric field and the chemically active species in the plasma. The PEFs are caused by applying pulse voltage between the electrodes and contribute to form pores on the cell membrane or to change conformation of protein. When the applied voltage exceeds the discharge onset criterion, plasmas are generated through the avalanche process of electron accelerated with intense electric field in a gas or liquids medium. .
8. Akiko Maruyama-Nakashita, Yohei Ishibashi, Kyotaro Yamamoto, Liu Zhang, Tomomi Morikawa-Ichinose, Sun-Ju Kim, Nobuya Hayashi, Oxygen plasma modulates glucosinolate levels without affecting lipid contents and composition in Brassica napus seeds, Bioscience, Biotechnology, and Biochemistry, https://doi.org/10.1093/bbb/zbab157, 85, 12, 2434-2441, 2021.12.
9. K Takahashi, N Hayashi, Plasma Chemical Reactions, Journal of The Institute of Electrical Engineers of Japan, 10.1541/ieejjournal.141.155, 141, 3, 155-158, 2021.03.
10. Sitti Subaedah Sahabuddin, Nobuya Hayashi, Growth Comparison Between Lymphocyte BW5147 T-cells and EL4 T-cells Using Atmospheric Oxygen Plasma Irradiation, Plasma Medicine, in press, 2021.04.
11. Sayma Khanom, Nobuya Hayashi, Removal of Metal Ions from Water Using Oxygen Plasmas, Scientific Reports, https://doi.org/10.1038/s41598-021-88466-3, 11, 9175, 2021.04, Zinc ion dissolved in water is attempted to be removed by generating the oxides of zinc using the oxygen gas in DBD plasma system. The removal rate of zinc oxides’ production (ZnO and Zn (OH2)) were measured at different treatment periods by the oxygen plasma penetration in water. The removal rate of the deposit increases initially and then decreases with the treatment period. The maximum removal rate (29%) of zinc from water is achieved at the treatment period of 10 min, where pH is minimum. From FTIR the generation properties of zinc oxide can be recognized. Initially the amount the deposit increases with the ozone treatment period due to production of both ZnO and Zn (OH) 2. After that, the production of Zn (OH) 4 2-increases even when the total removal rate of the deposit decreases. Therefore, to remove zinc ion from water forming metal oxide deposit, the penetration amount of the active oxygens to the water must be controlled to keep the pH lower than around 7.5. Because with increasing pH amount of removal rate of ZnO deposit decreases. The pH of the zinc dissolved water treated by ozone depends on both zinc and ozone concentration in water..
12. Norrawit Tonmitr, Takahiko Mori, Masashi Takami, Akira Yonesu, Nobuya Hayashi, Time-Modulated LF-Microwave Hybrid Plasma for Surface Sterilization, IEEE Transactions on Plasma Science, 10.1109/TPS.2020.3016378, 49, 1, 154-161, 2020.08, The plasma surface sterilization method has emerged as a new sterilization method for medical instruments with many advantages, including nontoxicity, short treatment time, and low thermal damage to the sterilized material, over conventional sterilization methods. In this study, low-frequency (LF)-microwave hybrid plasma is produced by irradiation of microwave to an LF plasma jet. This hybrid plasma is characterized by atmospheric-pressure nonthermal plasma with a large amount of reactive species and has been applied for plasma surface sterilization. To reduce the treatment temperature while maintaining the effect of surface sterilization for applicability to heat-sensitive materials, the LF-microwave hybrid plasma was modulated in time by using pulsed microwaves..
13. Koichi Takaki, Takayuki Ohshima, Nobuya Hayashi, Douyan Wang, Chunhong Yuan, Katsuyuki Takahashi, Yield and freshness improvement of fruits and vegetables by pulsed power plasma, Impact, https://doi.org/10.21820/23987073.2020.2.27, 2020, 2, 27-29, 2020.04, Growing populations, a changing climate, decreasing amounts of farmland and lower fertility soils are just a few of the many problems facing the world's food supply. Because of these multitude of changes, the agricultural industry and researchers recognise a dire need for more sustainable agriculture practices and technologies. Novel technologies and methods of improving crop yields while reducing waste throughout the food supply chain are two ways that we can reduce the pressure on our already stretched food supplies. A more sustainable and less wasteful system can also go a long way to providing higher quality goods to the segments of society that do not have the luxury of shopping at continually stocked, modern grocery stores. The methods used to tackle this problem vary, from increasing soil fertility via improved management practices to genetically modifying agriculture products themselves..
14. Minoru Noda, Yoshinobu Sakai, Yoshiro Sakaguchi, Nobuya Hayashi, Evaluation of low-temperature sterilizer using peracetic acid-containing hydrogen peroxide gas, Biocontrol Science, 2020.10.
15. Sitti Subaedah Sahabuddin, Haruka Ueamatsu, Nobuya Hayashi, Activation of EL-4 T-cells by irradiation with atmospheric oxygen plasma, Japanese Journal of Applied Physics, https://doi.org/10.35848/1347-4065/ab83db, 59, SJ, SJJF03, 2020.04, The activation of EL-4 T-cells is investigated when the T-cells are sensitized with monoclonal antibodies against CD3/CD28 followed by irradiation with atmospheric oxygen plasma. The proliferation of anti-CD3/CD28-sensitized EL-4 T-cells was evaluated as a function of the oxygen plasma irradiation duration. The plasma irradiation promotes cell proliferation: when plasma irradiation was applied to sensitize T-cells for 40 s, the cell number increased by five times over 24 h compared with that in a cell population that was not exposed to irradiation. Also, the plasma irradiation increases the production of interferon gamma in T-cells by a factor of two. Gene expression analysis of T-cell supports results of the proliferation and the immune function enhancements..
16. N. Hayashi, Y. Inoue, Y. Kyumoto, T. Kukita, Characteristics of differentiation of osteoclast cells irradiated with active species in atmospheric oxygen plasma, Japanese Journal of Applied Physics, https://doi.org/10.35848/1347-4065/ab7ba9, 59, SJ, SJJF02, 2020.04, Variations in osteoclast cell number are observed when osteoclast precursor cells are irradiated with atmospheric dielectric barrier discharge plasma. Active species generated by the oxygen plasma control the differentiation function of the osteoclast precursor cells. Long-lifetime active species such as H 2 O 2 and NO x–dissolved in the culture medium decrease the osteoclast number due to the inactivation of the differentiation function of the osteoclast precursor cells. When short-lifetime active species such as O* and OH* make contact with the osteoclast precursor cells directly, the osteoclast number tends to increase. Short-lifetime active species induce the enhancement of the gene expression of NFATc1..
17. N. Hayashi, Y.C. Yao, Y. Matsunaga, Regulation of macrophage-like cell activity driven by atmospheric oxygen plasma, Japanese Journal of Applied Physics, https://doi.org/10.35848/1347-4065/ab72cf, 59, SH, SHHF03, 2020.03, Oxygen plasma that is a kind of external biological stimulus activates the macrophage-like cell. Active oxygen species in the oxygen plasma-irradiated macrophage-like cells in culture medium to investigate the effects of different treatment periods and applied voltages. Macrophage-like cells were activated and increased 10% after 48 h from the short period plasma irradiation of 30 s. The long period irradiation for 60 and 120 s decreased the cell number by almost 33%. The phagocytosis capability increased by 1.5 times during the shorter plasma irradiation period, which would be owing to the increase of active oxygen species inside the macrophage cells..
18. Norrawit Tonmitr, Akira Yonesu and Nobuya Hayashi, Effect of gas composition on surface sterilization by using LF-microwave hybrid plasma source, Japanese Journal of Applied Physics, https://doi.org/10.7567/1347-4065/ab4614, 59, SA, SAAB02-1-SAAB02-6, 2019.11.
19. Takaki, Koichi, Hayashi, Nobuya, Wang, Douyan, Ohshima Takayuki, High-voltage technologies for agriculture and food processing, Journal of Physics D, 2019.10.
20. Nobuya Hayashi, Yukie Miyamaru, Reona Aijima, Yoshio Yamashita, Activation of p53-Mediated Apoptosis Pathway in HSC3 Cancer Cell Irradiated by Atmospheric DBD Oxygen Plasma, IEEE Transactions on Plasma Science, 10.1109/TPS.2018.2867431, 47, 2, 1093-1099, 2019.02, [URL], Oral cancer cell HSC3 is inactivated by the direct irradiation of oxygen plasma generated by torch-type oxygen dielectric barrier discharge. Selective inactivation of the cancer cells is observed at the discharge voltage of 4.2 kV, where normal cells suffer no damages from the plasma. Inactivation mechanism of HSC3 cells irradiated by the plasma is considered as the apoptosis. Activation of mitogen-activated protein kinase and p53 proteins owing to the phosphorylation is one of the pathways to the cancer cell apoptosis..
21. 中野 陸,林 信哉,合島怜於奈,山下佳雄,小林 明, 低圧酸素プラズマの照射による植物種子の遺伝子発現効果, Journal of IAPS, 26, 2, 91-95, 2018.12.
22. Tatsuya Nishikawa, Norihito Abe, Akira Yonesu and Nobuya Hayashi, Sterilization of Small Vial Using Electron Cyclotron Resonance Plasma, Vacuum, in press., 2018.10, The sterilization of a small vial using electron cyclotron resonance (ECR) plasma was investigated. The ECR plasma was generated throughout the inside of the small vial, which was sealed with Tybek sheet, by using magnetic configuration in which the ECR point was located inside the small vial. Moreover, it was demonstrated that the temperature of the small vial was finely controlled by the pulse width and pulse frequency of the pulsed microwave power. A biological indicator (BI), Geobacillus Stearothermophilus, containing 1×10^5 spores present inside the small vial was successfully inactivated in a total treatment time of about 14 min while maintaining treatment temperature of less than 65 ºC..
23. 天羽 仁美,安楽 大輝,板良敷 朝将,谷口 暢,林 信哉, 過酢酸含有過酸化水素ガス滅菌器の芽胞不活化効果, 医療機器学会誌, in press., 2018.06, 過酢酸含有過酸化水素ガス滅菌器は, 滅菌剤として8 %過酸化水素水溶液[過酢酸含有(
24. Nobuya Hayashi, Masaaki Goto, Tomomasa Itarashiki, Akira Yonesu, Akikazu Sakudo, Current plasma sterilization and disinfection studies, Journal of Photopolymer Science and Technology, in press., 2018.06, Plasma sterilization methods have been studied for over 20 years in Japan and around the world, and are currently being developed for practical application. However, persisting challenges, such as the speed of the sterilization treatment, treatment of pathogenic proteins on medical equipment, and the compatibility of plasma with materials of medical equipments, currently limit the widespread application of the techniques. This paper introduces research into plasma sterilization for practical use, which is currently being carried out..
25. 田中 大,林 信哉,野田 稔,酒井義信,後藤昌昭,花田康史,荒木真二, 各種滅菌法による樹脂製テストピースの強度変化と表面劣化についての評価, 医療機器学会, 87, 2017.10.
26. Reoto Ono, Shohei Uchida, Nobuya Hayashi, Rina Kosaka and Yasutaka Soeda, Inactivation of bacteria on plant seed surface by low-pressure RF plasma using a vibrating stirring device, Vacuum, 10.1016/j.vacuum.2016.07.017, 136, 214-220, 2017.07.
27. Keisuke Mine, Yukie Miyamaru, Nobuya Hayashi, Reona Aijima, Yoshio Yamashita, Mechanism of inactivation of oral cancer cells irradiated by active oxygen species from DBD plasma, Plasma Medicine Journal, 10.1615/PlasmaMed.2017019246, 7, 201-213, 2017.07.
28. Akira Yonesu, Kazunori Koga, Masaharu Shiratani, Nobuya Hayashi, Influence of plasma irradiation on silkworm, Plasma Medicine, 7, 4, 313-320, 2017.01, Silkworms have recently been proposed as an animal model for safety testing in basic research. We propose using silkworms for in vivo trials of direct plasma treatment. In this study, the influence of plasma irradiation on silkworms was investigated using a non-thermal atmospheric pressure plasma. Silkworm survival rate decreased with increasing low-frequency voltage and plasma irradiation period. Further investigation of the plasma-generated agents (oxygen related radicals, UV light, and charged particles), revealed that the contribution of charged particles significantly increases silkworm mortality..
29. Nobuya Hayashi, Reoto Ono, Riku Nakano, Masaharu Shiratani, Kosuke Tashiro, Satoru Kuhara, Kaori Yasuda, and Hiroko Hagiwara, DNA microarray analysis of plant seeds irradiated by active oxygen species in oxygen plasma, Plasma Medicine Journal, DOI: 10.1615/PlasmaMed.2016018933, 6, 459-471, 2016.12.
30. Thapanut Sarinont, Takaaki Amano, Pankaj Attri, Kazunori Koga, Nobuya Hayashi, Masaharu Shiratani, Effects of ambient gas of plasma irradiation to seeds of Raphanus sativus L. on their growth, Archives of Biochemistry and Biophysics , in press, 2016.10.
31. Thapanut Sarinont, Takaaki Amano, Pankaj Attri, Kazunori Koga, Nobuya Hayashi, Masaharu Shiratani, Effects of Plasma Irradiation using Various Feeding Gases on Growth of Raphanus sativus L., Archives of Biochemistry and Biophysics, in press, 2016.10.
32. Riku Nakano, Kosuke Tashiro, Reona Aijima, and Nobuya Hayashi, Effect of oxygen plasma irradiation on gene expression in plant seeds induced by active oxygen species, Plasma Medicine Journal, DOI: 10.1615/PlasmaMed.2016019093, 6, 303-313, 2016.10.
33. Satoshi Watanabe, Reoto Ono, Nobuya Hayashi, Kosuke Tashiro, Satoru Kuhara, Asami Inoue, Kaori Yasuda, Hiroko Hagiwara, Growth Enhancement and Gene Expression of Arabidopsis irradiated by active oxygen species, Japanese Journal of Applied Physics, 55, 8, 2016.08.
34. Yasuhiro Sakai, Zhen Liu, Nobuya Hayashi, Sterilization characteristics of dental instruments using Oxygen Plasma produced by narrow gap RF discharge, Japanese Journal of Applied Physics, 55, 8, 2016.08.
35. Yagyu Yoshihito, Hatayama Yuuta, Hayashi Nobuya, Mishima Tomoko, Nishioka Terumi, Sakudo Akikazu, Ihara Takeshi, Ohshima Tamiko, Kawasaki Hiroharu, Suda Yoshiaki, Direct Plasma Disinfection of Green Mold Spore on Citrus by Atmospheric Pressure Dielectric Barrier Discharge for Agricultural Applications, Trans. Mat. Res. Soc. Japan, 10.14723/tmrsj.41.127, 41, 127-130, 2016.07.
36. 野田 稔, 酒井 義信, 坂口 嘉郎, 後藤 昌昭, Nobuya Hayashi, 過酸化水素ガスを用いた低温滅菌装置の滅菌性能について, 医療機器学, 86, 604-611, 2016.06.
37. 林 信哉,柳生義人, 高電圧・プラズマによる農業応用の基礎, 電気学会誌, 10.1541/ieejjournal.136.798, 136, 798-801, 2016.06.
38. Tomomasa Itarashiki, Nobuya Hayashi, Akira Yonesu, Characteristics of plasma sterilizer using microwave torch plasma with AC high-voltage discharge plasma, Jpn. J. Appl. Phys., 10.7567/JJAP.55.01AB03, 55, 01AB03-1-01AB03-6, 2016.01.
39. Kazunori Koga, Nobuya Hayashi, Takaaki Amano, Naho Itagaki, Masaharu Shiratani, A simple method to improve harvest by non-thermal air plasma irradiation to seeds of Arabidopsis thaliana L., Appl. Phys. Exp., 10.7567/APEX.9.016201, 9, 016201-1-016201-3, 2016.01, シロイヌナズナ(Arabidopsis thaliana)の種子への非熱平衡プラズマ照射が、栽培開始から収穫までの成長に及ぼす影響を調べた。プラズマを乾燥種子へ3分間照射した結果、すべての成長段階において成長促進をもたらした。プラズマ未照射種子と比較してプラズマ照射種子は収穫期間が11%短縮し、種子の重量が56%増加し、種子数が39%増加することが分かった。.
40. Tomomasa Itarashiki, Nobuya Hayashi, Akira Yonesu, Decomposition of Proteins Using a Microwave Air Plasma Sterilizer, Trans. Mat. Res. Soc. Japan, 10.14723/tmrsj.41.179, 41, 179-182, 2016.01.
41. 小野大帝, 林 信哉, 低圧酸素プラズマ照射による植物の抗酸化特性の評価, 電気学会, 135, 6, 347-352, 2015.06.
42. Reoto Ono, Nobuya Hayashi, Variation of antioxidative activity and growth enhancement of Brassicaceae induced by low-pressure oxygen plasma, Japanese Journal of Applied Physics , 10.7567/JJAP.54.06GD03, 54, 6, 06GD03-1- 06GD03-4, 2015.06.
43. Hayashi Nobuya, Reoto Ono, Shohei Uchida, Growth Enhancement of Plant by Plasma and UV Light Irradiation to Seeds, J. Photopolym. Sci. Technol, 28, 445-448, 2015.06.
44. Hayashi Nobuya, Ono Reoto, Yonesu Akira, Antioxidative activity of plant and regulation of Brassicaceae induced by oxygen radical irradiation, Japanese Journal of Applied Physics, 10.7567/JJAP.54.06GD01, 54, 06GD01-1-06GD01-6, 2015.05.
45. 林 信哉, 小野大帝, 大気圧プラズマの農水産物応用, 日本AEM学会, 22, 447-452, 2014.12.
46. Itarashiki Tomomasa, Hayashi Nobuya, Yonesu Akira, Sterilization effect of nitrogen oxide radicals generated by microwave plasma using air, VACUUM, 10.1016/j.vacuum.2014.06.018, 110, 217-220, 2014.12.
47. Kitazaki Satoshi, Tanaka Akimasa, Hayashi Nobuya, Sterilization of narrow tube inner surface using discharge plasma, ozone, and UV light irradiation, VACUUM, 10.1016/j.vacuum.2014.06.014, 110, 217-220, 2014.12.
48. 林 信哉, 門脇一則, 高木浩一, 王 斗艶, 西村 亮, パルスパワー・プラズマによる農作物の収量改善, プラズマ・核融合学会, 90, 541-546, 2014.09.
49. 小野大帝, 渡辺風子, 林 信哉, プラズマを用いた医療用滅菌法のフロンティア, 日本機械学会, 117, 470-473, 2014.07.
50. Kitazaki Satoshi, Sarinont Thapanut, Kazunori Koga, Hayashi Nobuya, Masaharu Shiratani, Plasma induced long-term growth enhancement of Raphanus sativus L. using combinatorial atmospheric air dielectric barrier discharge plasmas, CURRENT APPLIED PHYSICS, 10.1016/j.cap.2013.11.056, 14, S149-S153, 2014.07.
51. 小野大帝, 林 信哉, 酸素プラズマ中で生成される活性酸素による植物成長促進の機序, プラズマ応用科学会, 22, 51-54, 2014.06.
52. Hayashi Nobuya, Yagyu, Yoshihito, Yonesu Akira, Masaharu Shiratani, Sterilization characteristics of the surfaces of agricultural products using active oxygen species generated by atmospheric plasma and UV light, JAPANESE JOURNAL OF APPLIED PHYSICS, 10.7567/JJAP.53.05FR03, 53, 5, 05FR03-1-05FR03-5, 2014.05.
53. T. Sarinont, T. Amano, Hayashi Nobuya, Kazunori Koga, Masaharu Shiratani, Growth enhancement effects of radish sprouts: atmospheric pressure plasma irradiation vs. heat shock, Journal of Physics, 10.1088/1742-6596/518/1/012017, 518, 012017-1-012017-6, 2014.03.
54. T. Sarinont, Hayashi Nobuya, Kazunori Koga, Masaharu Shiratani, Effects of Atmospheric Air Plasma Irradiation on pH of Water, Journal of Physics Society, 10.7566/JPSCP.1.015078, 1, 015078-1-015078-4, 2014.03.
55. Reoto Ono, Akimasa Tanaka, Shohei Uchida, Tomomasa Itarashiki, Hayashi Nobuya, Effect of Active Oxygen Species in Low Pressure Oxygen Plasma on Antioxidative Substances, Institute of Applied Plasma Sciences, 7, 45-46, 2014.01.
56. S. Kitazaki, Kazunori Koga, Masaharu Shiratani, Hayashi Nobuya, Growth Control of Dry Yeast Using Scalable Atmospheric Pressure Dielectric Barrier Discharge Plasma Irradiation, Jpn. J. Appl. Phys., 51, 11PJ02, 2013.01.
57. Hayashi Nobuya, Yusuke Akiyoshi, Yasuyo Kobayashi, Kohzo Kanda, Kazusato Ohshima, Masaaki Goto, Inactivation Characteristics of Bacillus Thuringiensis Spore in Liquid Using Atmospheric Torch Plasma Using Oxygen, Vacuum, 88, 173-176, 2013.01.
58. Sakudo A, Hayashi Nobuya, Shimizu N, Shintani H, Degradation of Influenza Virus Nucleoprotein by N2 Gas Plasma, MRS Proc., 1469, mrss12-1469-ww06­08, 2012.10.
59. S Kitazaki, Kazunori Koga, Masaharu Shiratani, Hayashi Nobuya, Rapid Growth of Radish Sprouts Using Low Pressure O2 Radio Frequency Plasma Irradiation, MRS Proc., mrss12­1469­ww02­08, 2012.10.
60. S Kitazaki, Kazunori Koga, Masaharu Shiratani, Hayashi Nobuya, Effects of Atmospheric Pressure Dielectric Barrier Discharge Plasma Irradiation on Yeast Growth, MRS Proc., mrss12­1469­ww0608, 2012.10.
61. Y. Akiyoshi, Hayashi Nobuya, S. Kitazaki, Kazunori Koga, Masaharu Shiratani, Influence of Atmospheric Pressure Torch Plasma Irradiation on Plant Growth, MRS Proc., 1469, mrss12-1469-ww06-10, 2012.10.
62. 林 信哉, 米須 章, プラズマの農業応用 農産物殺菌から植物成長制御まで, 電気学会誌, 132, 702-705, 2012.07.
63. Satoshi Kitazaki, Kazunori Koga, Masaharu Shiratani, Hayashi Nobuya, Growth Enhancement of Radish Sprouts Induced by Low Pressure O2 RF Discharge Plasma Irradiation, Jpn. J. Appl. Phys., 10.1143/JJAP.51.01AE01, 51, 01AE01-1 - 01AE01-4, 2012.01.
64. 山下佳雄,木戸淳太,後藤昌昭,林 信哉, 酸素ラジカルを用いた低温プラズマ滅菌装置の歯科材料に対する滅菌性能, 日本口腔科学会雑誌, 61, 1, 16-23, 2012.01.
65. Nobuya Hayashi, Takaomi Nakashima, Akira Yonesu, Sterilization of Medical Equipments Using Air Torch Plasma Produced by Microwave Discharge, IEEE Trans. on Plasma Science, 39, 2976-2977, 2011.12, Sterilization of non-heat-proof equipments was
performed by irradiation from air torch plasma produced by
microwave discharge. The downstream region of the torch
plasma exhibits a yellowish color. Light emission spectra and chemical indicators show that nitrogen oxide radicals are the origin of this color. Inactivation of spore-type Geobacilius stearothermophilus with the population of 105 was successful for a treatment period of 10 minutes..
66. Nobuya Hayashi, Akari Nakahigashi, Masaaki Goto, Satoshi Kitazaki, Kazunori Koga, Masaharu Shiratani, Redox Characteristics of Thiol Compounds Using Radicals Produced by Water Vapor RF Discharge, Jpn. J. Appl. Phys., 50, 08JF04-1 - 08JF04-5, 2011.08.
67. Nobuya Hayashi, Akari Nakahigashi, Hao LIU, Masaaki Goto, Treatment of second order structures of protein using oxygen RF plasma, Jpn. J. Appl. Phys., 49, 08JH02-1 - 08JH02-4, 2010.08.
68. Yoshihito Yagyu, Akira Hikida, Nobuya Hayashi, Hiroharu Kawasaki, Tamiko Ohshima and Yoshiaki Suda, Studies on Allergic Substance Elimination by RF Plasma Treatment, J. Materials Research, 35, 119-122, 2010.06.
69. 酒井義信,野田 稔,後藤昌昭,林 信哉, 滅菌の確認法-インジケータの活用, 感染症対策ICTジャーナル, 5, 60-66, 2010.04.
70. Nobuya HAYASHI, Ryutaro KAWAGUCHI and Hao LIU, Treatment of Protein Using Oxygen Plasma Produced by RF Discharge, J. Plasma and Fusion Research, 8, 552-555, 2009.04.
71. Weimin GUAN, Hiroharu KAWASAKI, Tamiko OHSHIMA, Yoshihito YAGYU, Toshinobu SHIGEMATSU Yoshiaki SUDA and Nobuya HAYASHI, Influence of Calcium Hydroxide Solution in RF Plasma on Sterilization of Bacterial Spores, J. Plasma and Fusion Research, 8, 573-577, 2009.04.
72. Yoshihito YAGYU, Nobuya HAYASHI, Weimin GUAN, Hiroharu KAWASAKI, Influence of Atomic and Singlet Molecular Oxygen Generated by RF Plasma on Reduction of Protein, J. Plasma and Fusion Research, 8, 578-581, 2009.04.
73. Nobuya Hayashi and Yoshihito Yagyu, Treatment of protein using oxygen plasma produced by RF discharge, Trans. of the Material Research Society of Japan, 33, 791-794, 2008.10.
74. Satoshi Kitazaki and Nobuya Hayashi, Sterilization characteristics of tube inner surface using oxygen plasma produced by AC HV discharge, IEEE Trans. on Plasma Science, 36, 1304-1305, 2008.10.
75. Nobuya Hayashi, Shinsuke Tsutsui, Tetsushi Tomari, Weimin Guan, Sterilization of Medical Equipments Using Oxygen Radicals Produced by Water Vapor RF Plasma, IEEE Trans. on Plasma Science, 36, 1302-1303, 2008.10.
76. Nobuya Hayashi, Shohei Sougumo, Suppression of by-product generation in the treatment of aromatic perfumery substances using a surface discharge, Vacuum, 83, 138-141, 2008.09.
77. 林 信哉,米須 章, マイクロ波トーチプラズマによる表面滅菌処理の基礎特性, ケミカルエンジニヤリング, 53, 51-54, 2008.06.
78. Y. Yagyu, N. Hayashi, H. Kawasaki, T. Ohshima, Y. Suda and S. Baba, Fundamental Studies on Effect of Ozone Injection to the Internal-Combustion Engine – FTIR spectrum of Hydrocarbon Compound Reformulated by Ozone –, Journal of Physics: Conf. Ser., 100, 1-4, 2008.04.
79. Nobuya Hayashi, Weimin Guan, Shinsuke Tsutsui, Tetsushi Tomari, Yasushi Hanada, Sterilization characteristics for medical equipments using radicals produced by oxygen/water vapor RF plasma, Jpn. J. Appl. Phys., 45, 8358-8363, 2006.12.
80. Nobuya Hayashi, Tsutomu Yamakawa, Seiji Baba, Effect of additive gases on synthesis of organic compounds from carbon dioxide using non-thermal plasma produced by atmospheric surface discharges, Vacuum, 80, 1299-1304, 2006.06.
81. S. K. Hong, N. Hayashi, S. Ihara, S. Satoh, Formation properties of the main-discharge in pure Ar gas using the automatically pre-ionized plasma electrode, IEEE Trans. on Plasma Science, 33, 324-325, 2005.08.
82. Nobuya Hayashi, Saburoh Satoh, Treatment of Perfluorocarbon Using Nonthermal Plasma Produced by Atmospheric Streamer Corona Discharge, IEEE Trans. on Plasma Science, 33, 274-275, 2005.08.
83. Fumiaki Takeshita, Tomoaki Miichi, Nobuya Hayashi, Water treatment using discharge on the surface of a bubble in water, Plasma Processes and Polymers, 2, 246-251, 2005.04.
84. S. K. Hong, N. Hayashi, S. Ihara, S. Satoh, Main-discharge formation and light emission in pure Ar gas at multi-atmospheric pressure using the automatically pre-ionized plasma electrode, Vacuum, 79, 25-36, 2005.01.
85. M. Yamaura, N. Hayashi, S. Ihara, S. Satoh, Lifetime evaluation of weakly ionized plasma channel by accumulation effect of charged particles by means of laser absorption, J. Appl. Phys., 95, 6007-6010, 2004.12.
86. Mitsuaki Shimosaki, Nobuya Hayashi, Satoshi Ihara, Saburoh Satoh, Effect of trigger electrode configuration of a double discharge ozonizer on ozone generation characteristics, Vacuum, 73, 573-578, 2004.09.
87. Shunichi Kaneda, Nobuya Hayashi, Satoshi Ihara, Saburoh Satoh, Application of dielectric material to double-discharge-type ozonizer, Vacuum, 73, 567-571, 2004.09.
88. Joanna Pawlat, Nobuya Hayashi, Satoshi Ihara, Foaming Column with a Dielectric Covered Plate-to-Metal Plate Electrode as an Oxidants Generator, Advances in Environmental Research, 8, 351-358, 2004.06.
89. Hee-Sung Ahn, Nobuya Hayashi, Satoshi Ihara, Ozone Generation Characteristics by Superimposed Discharge in Oxygen- Fed Ozonizer, Jpn. J. Appl. Phys., 42, 6578-6583, 2003.11.
90. Joanna Pawlat, Nobuya Hayashi, Satoshi Ihara and Iwo Pollo, Studies on Oxidants, Generation in a Foaming Column with a Needle to Dielectric Covered Plate Electrode, 23, 569-583, 2003.09.
91. Joanna Pawlat, Nobuya Hayashi, Satoshi Ihara, Generation of Discharge in Dynamic Foam for Oxidants Formation Using Various Type of Electrode, Colloids and Surfaces A: Physicochem. Eng. Aspects, 220, 1-12, 2003.06.
92. Joanna Pawlat, Nobuya Hayashi, Satoshi Ihara, Studies on Oxidants’ Generation in a Forming Column with a Needle to Dielectric Cover Plate Electrode, Plasma Chemistry and Plasma Processing, 3, 567-581, 2003.05.
93. Michiteru Yamaura, Nobuya Hayashi, Satoshi Ihara, Saburoh Satoh, Evaluation of Weakly Ionized Plasma Channel by Accumulation Effect of Charged Particles Using High-Repettion-Rate KrF Excimer Laser, Jpn. J. Appl. Phys., 41, 6394-6397, 2002.11.
94. N. Hayashi, K. Yamamoto, S. Ihara, S. Satoh and C. Yamabe, Treatment of Fluorocarbon Using Nonthermal Plasma Produced by Atmospheric Discharge, Jpn. J. Appl. Phys., 41, 5399-5403, 2002.08.
95. Tomoaki Miichi, Nobuya Hayashi, Satoshi Ihara, Saburoh Satoh and Chobei Yamabe, Generation of Radicals using Discharge inside Bubbles in Water for Water Treatment, Ozone Science and Engineering, 24, 471-477, 2002.06.
96. Penghui Guan, Nobuya Hayashi, Saburoh Satoh, NOx Treatment by DC Streamer Corona Discharge with Series Gap, Vacuum, 65, 469-474, 2002.05.
97. Joanna Pawlat, Nobuya Hayashi, Chobei Yamabe and Iwo Pollo, Generation of oxidants with a foaming system and it’s electrical properties, Ozone Science and Engineering, 24, 181-191, 2002.03.
98. Karol Hensel, Nobuya Hayashi, Chobei Yamabe and Marcela Morvová, Positive DC Corona Discharge in N2–NO–CO2–O2 Mixtures, Jpn. J. Appl. Phys., 41, 336-345, 2002.01.
99. Saburoh Satoh, Nobuya Hayashi, Satoshi Ihara, Chobei Yamabe and Takio Tomimasu, Outlines of Saga FEL project, Jpn. J. Appl. Phys., 41, 41-43, 2002.01.
100. Joanna Pawlat, Nobuya Hayashi, Chobei Yamabe, Studies on Electrical Discharge Effects in a Foaming Environment, Jpn. J. Appl. Phys., 40, 12, 7061-7066, 2001.12.
101. Nobuya Hayashi, Hideo Suganuma, Masaki Kamatani, Saburoh Satoh, Treatment of Volaile Organic Compound by Positive Streamer Corona using a Series Gap, Jpn. J. Appl. Phys., 40, 10, 6104-6108, 2001.10.
102. 山浦道照,林 信哉,猪原 哲,佐藤三郎,山部長兵衛, 高繰り返しKrFエキシマレーザを用いた荷電粒子蓄積効果による弱電離プラズマチャネルの検討, 電気学会論文誌A, 121-A, 828-833, 2001.09.
103. N. Hayashi, Observation of Submicron Dust Particles Trapped in a Diffused Region of a Low Pressure Radio Frequency Plasma, Physics of Plasmas, 8, 6, 3051-3057, 2001.06, Submicron dust particles (Cu, ϕ 50 nm) were observed to be trapped in an rf plasma at relatively low pressure. The laser light scattering by particles shows the time evolution of the dust particles for several hours after pulsed injection of the particles. The dust particles were localized in the diffused region as a large volume cloud. The suspension mechanism of the dust particles in the diffused plasma was explained by an electrostatic force due to the potential structure. The spatial distribution of the charge of the dust particles was determined by this potential structure. The dispersion relations of the self-excited dust acoustic waves were adopted to confirm the spatial distribution and the time evolution of the charge of the dust particles. .
104. 見市知昭,林 信哉,猪原 哲,佐藤三郎,山部長兵衛, 水中気泡内放電によるオゾン生成, 電気学会論文誌A, 121-A, 448-452, 2001.05.
105. N. Hayashi, T. Nakashima and H. Fujita, Reduction of Electron Temperature in rf Plasma Using Magnetic Filter, Jpn. J. Appl. Phys., 38, 4301-4304, 1999.07.
106. K. Taniguchi, H. Kuwae, N. Hayashi, Y. Kawai, Observation of type-1 intermittency caused by current-driven ion acoustic instability, Physics of Plasmas, 10.1063/1.872724, 5, 2, 401-405, 1998.05, Intermittent chaotic phenomena caused by the current-driven ion acoustic instability are experimentally observed using a Double Plasma device, in which two mesh grids are installed to excite the instability. When a dc potential is applied to one of the two mesh grids and exceeds a certain threshold, the system suddenly transits from a periodic state to a chaotic state. At the same time, the signals picked up as perturbation components of a current exhibit intermittent turbulent bursts. The calculations of the correlation dimension and the Lyapunov exponent indicate that the system reaches a chaotic state. Furthermore, it is found that the results of mathematical and statistical analysis of observed signals agree with the theoretical properties of the type-1 intermittency: the occurrence of 1/f-type low-frequency noise and the probability distribution of the duration between two bursts. Therefore, it is concluded that the present system reaches a chaotic state via the type-1 intermittency. .
107. 河合良信、林 信哉, 電子ビーム・プラズマ系における分岐現象, プラズマ核融合学会誌, 73, 10, 1111-1117, 1997.10,
Bifurcation phenomena are experimentally observed in an electron beam plasma system using a double plasma device. Upon injection of an electron beam into the target plasma, both an unstable wave and subharmonics are observed. The fundamental unstable wave is classified as to be a beam mode wave excited by electron beam plasma instability. It is confirmed that these bifurcation phenomena originate from nonlinearity of the unstable wave.

.
108. N. Hayashi and Y. Kawai, Observation of bifurcation phenomena in an electron beam plasma system, Physics of Plasmas, 10.1063/1.872062, 3, 12, 4440-4445, 1996.12, Bifurcation phenomena are experimentally observed in an electron beam plasma system using a double plasma device. When an electron beam is injected into the target plasma, an unstable wave and the subharmonics of period 2, period 3, and period 4 are observed. The fundamental unstable wave is specified to be a beam mode wave excited by an electron beam plasma instability. It is confirmed that these bifurcation phenomena originate from nonlinearity of the unstable wave. The correlation dimensions and Lyapunov exponents indicate that the system becomes chaotic when the subharmonics of period 3 or period 4 appear. .
109. N. Hayashi, M. Tanaka, S. Shinohara, and Y. Kawai, Excitation mechanism of standing waves produced by electron beam plasma instability, Physics of Plasmas, 10.1063/1.871057, 2, 10, 3582-3587, 1995.10, The excitation mechanism of standing waves produced by the electron beam plasma instability is experimentally studied using a Double Plasma device. When an electron beam is injected into the target plasma, standing waves around the electron plasma frequency are excited. A test wave is propagated in an electron beam plasma system and is identified as the beam mode from the dispersion relation. The propagation direction of the beam mode is determined from the wave pattern utilizing a phase shifter. It is found that a reflected beam mode exists as well as a forward beam mode, and is generated by the reflection of the forward beam mode from a potential well produced by the injection of the electron beam. The observed standing waves are formed by superposing the beam modes propagating in opposite directions from each other. .

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